Detailed information NTG0352396

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GFX43_RS13000
• Genome accession: NZ_CP046448
• Coordinates: 2555991..2556980 (-)
• Length: 329 a.a.
• NCBI ID: WP_015383647.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain ZD01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2550991..2561980

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFX43_RS12960 (GFX43_012960)	trmD	2551218..2551949 (-)	732	WP_015383650.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
GFX43_RS12965 (GFX43_012965)	rimM	2551946..2552470 (-)	525	WP_003232013.1	ribosome maturation factor RimM	-
GFX43_RS12970 (GFX43_012970)	ylqD	2552475..2552861 (-)	387	WP_015383649.1	YlqD family protein	-
GFX43_RS12975 (GFX43_012975)	khpA	2552983..2553228 (-)	246	WP_014476792.1	KH domain-containing protein	-
GFX43_RS12980 (GFX43_012980)	rpsP	2553228..2553500 (-)	273	WP_003220815.1	30S ribosomal protein S16	-
GFX43_RS12985 (GFX43_012985)	ffh	2553606..2554946 (-)	1341	WP_014113771.1	signal recognition particle protein	-
GFX43_RS12990 (GFX43_012990)	srpM	2554960..2555292 (-)	333	WP_003238590.1	putative DNA-binding protein	-
GFX43_RS12995 (GFX43_012995)	sivC	2555470..2555955 (+)	486	WP_003245834.1	Ig-like domain-containing protein	-
GFX43_RS13000 (GFX43_013000)	pilA	2555991..2556980 (-)	990	WP_015383647.1	signal recognition particle-docking protein FtsY	Machinery gene
GFX43_RS13005 (GFX43_013005)	smc	2557000..2560560 (-)	3561	WP_015383646.1	chromosome segregation protein SMC	-
GFX43_RS13010 (GFX43_013010)	rncS	2560661..2561410 (-)	750	WP_003232030.1	ribonuclease III	-
GFX43_RS13015 (GFX43_013015)	acpP	2561550..2561783 (-)	234	WP_003154310.1	acyl carrier protein	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36356.55 Da
• Isoelectric Point: 5.0164

>NTDB_id=352396 GFX43_RS13000 WP_015383647.1 2555991..2556980(-) (pilA) [Bacillus subtilis strain ZD01]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADILICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=352396 GFX43_RS13000 WP_015383647.1 2555991..2556980(-) (pilA) [Bacillus subtilis strain ZD01]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTTGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAAGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTTAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCAGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATATATTAATTTGTGATACGGCAGGTCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAGTTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

• SRP54-type protein, GTPase domain (SRP54) (121-320)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	44.04	91.793	0.404