Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E4F38_RS15425 Genome accession   NZ_CP038218
Coordinates   2957377..2958696 (+) Length   439 a.a.
NCBI ID   WP_004522862.1    Uniprot ID   Q63XM5
Organism   Burkholderia pseudomallei strain Yap5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2952377..2963696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4F38_RS15390 (E4F38_15385) - 2952897..2953511 (+) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -
  E4F38_RS15395 (E4F38_15390) pth 2953625..2954230 (+) 606 WP_004530939.1 aminoacyl-tRNA hydrolase -
  E4F38_RS15400 (E4F38_15395) hisC 2954319..2955389 (+) 1071 WP_004530940.1 histidinol-phosphate transaminase -
  E4F38_RS15405 (E4F38_15400) - 2955559..2955825 (-) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  E4F38_RS15410 (E4F38_15405) coaD 2955888..2956388 (-) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  E4F38_RS15420 (E4F38_15415) rsmD 2956499..2957125 (-) 627 WP_004522861.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  E4F38_RS15425 (E4F38_15420) pilA 2957377..2958696 (+) 1320 WP_004522862.1 signal recognition particle-docking protein FtsY Machinery gene
  E4F38_RS15430 (E4F38_15425) maiA 2958853..2959497 (-) 645 WP_004522863.1 maleylacetoacetate isomerase -
  E4F38_RS15435 (E4F38_15430) - 2959494..2959835 (-) 342 WP_009918580.1 hypothetical protein -
  E4F38_RS15440 (E4F38_15435) nirB 2959882..2962326 (+) 2445 WP_004537843.1 nitrite reductase large subunit NirB -
  E4F38_RS15445 (E4F38_15440) - 2962402..2962797 (+) 396 WP_004537842.1 nitrite reductase (NAD(P)H) small subunit -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46312.63 Da        Isoelectric Point: 6.2545

>NTDB_id=352362 E4F38_RS15425 WP_004522862.1 2957377..2958696(+) (pilA) [Burkholderia pseudomallei strain Yap5]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLGALREKVRAGRLTDPQQVKDALRGLLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=352362 E4F38_RS15425 WP_004522862.1 2957377..2958696(+) (pilA) [Burkholderia pseudomallei strain Yap5]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAGCCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGATGCGCTGCGCGGGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTTGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(240-439)

SRP54-type protein, helical bundle domain (SRP54_N)

(158-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q63XM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.113

100

0.506

Multiple sequence alignment