Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E4F38_RS15320 Genome accession   NZ_CP038218
Coordinates   2940332..2941840 (+) Length   502 a.a.
NCBI ID   WP_004195216.1    Uniprot ID   A0AAX1X7H0
Organism   Burkholderia pseudomallei strain Yap5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2935332..2946840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4F38_RS15295 (E4F38_15290) kdsD 2936564..2937547 (+) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -
  E4F38_RS15300 (E4F38_15295) - 2937547..2938086 (+) 540 WP_004200146.1 HAD family hydrolase -
  E4F38_RS15305 (E4F38_15300) lptC 2938092..2938706 (+) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  E4F38_RS15310 (E4F38_15305) lptA 2938725..2939390 (+) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  E4F38_RS15315 (E4F38_15310) lptB 2939387..2940163 (+) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  E4F38_RS15320 (E4F38_15315) rpoN/rpoN1 2940332..2941840 (+) 1509 WP_004195216.1 RNA polymerase factor sigma-54 Machinery gene
  E4F38_RS15330 (E4F38_15325) hpf 2941990..2942349 (+) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E4F38_RS15335 (E4F38_15330) ptsN 2942582..2943091 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E4F38_RS15340 (E4F38_15335) hprK 2943220..2944188 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  E4F38_RS15345 (E4F38_15340) rapZ 2944274..2945167 (+) 894 WP_004195227.1 RNase adapter RapZ -
  E4F38_RS15350 (E4F38_15345) - 2945194..2945826 (+) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54889.04 Da        Isoelectric Point: 4.8740

>NTDB_id=352361 E4F38_RS15320 WP_004195216.1 2940332..2941840(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain Yap5]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=352361 E4F38_RS15320 WP_004195216.1 2940332..2941840(+) (rpoN/rpoN1) [Burkholderia pseudomallei strain Yap5]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment