Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GNT61_RS01965 Genome accession   NZ_CP046405
Coordinates   425945..427291 (+) Length   448 a.a.
NCBI ID   WP_003160919.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5443     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420945..432291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNT61_RS01945 (GNT61_01935) - 421782..422348 (-) 567 WP_003084491.1 hypothetical protein -
  GNT61_RS01950 (GNT61_01940) rsmD 422351..422947 (-) 597 WP_003142626.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GNT61_RS01955 (GNT61_01945) - 422947..424434 (-) 1488 WP_003142627.1 pitrilysin family protein -
  GNT61_RS01960 (GNT61_01950) - 424427..425824 (-) 1398 WP_030047726.1 pitrilysin family protein -
  GNT61_RS01965 (GNT61_01955) pilA 425945..427291 (+) 1347 WP_003160919.1 signal recognition particle-docking protein FtsY Machinery gene
  GNT61_RS01970 (GNT61_01960) ftsE 427288..427959 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  GNT61_RS01975 (GNT61_01965) ftsX 427959..428966 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  GNT61_RS01980 (GNT61_01970) rpoH 429080..429934 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  GNT61_RS01985 (GNT61_01975) - 429999..430604 (+) 606 WP_003084511.1 hypothetical protein -
  GNT61_RS01990 (GNT61_01980) mtgA 430609..431340 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GNT61_RS01995 (GNT61_01985) - 431377..431754 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  GNT61_RS02000 (GNT61_01990) thiS 431857..432057 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 47646.03 Da        Isoelectric Point: 4.6083

>NTDB_id=351935 GNT61_RS01965 WP_003160919.1 425945..427291(+) (pilA) [Pseudomonas aeruginosa strain SE5443]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIGEG
MASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDVAR
EPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQAA
KARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLDGT
AKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=351935 GNT61_RS01965 WP_003160919.1 425945..427291(+) (pilA) [Pseudomonas aeruginosa strain SE5443]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGGCAGTCA
GCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGCATCGGCGAAGGC
ATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGACCGCCGA
CGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACAGCGGCG
CGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTCGCGCGC
GAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAAGCTGCA
ACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCTGGGGCG
AGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAGGCCGCC
AAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGAGCTGAA
GAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCACCGGGC
AGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGACGGCACC
GCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGGGATCGA
CGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-223)

SRP54-type protein, GTPase domain (SRP54)

(243-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.907

95.982

0.46

Multiple sequence alignment