Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E2544_RS00695 Genome accession   NZ_CP038034
Coordinates   148685..150130 (+) Length   481 a.a.
NCBI ID   WP_063961223.1    Uniprot ID   -
Organism   Achromobacter insolitus strain LCu2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 143685..155130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2544_RS00680 (E2544_00680) - 144404..145732 (+) 1329 WP_042795749.1 amidase -
  E2544_RS00685 (E2544_00685) leuA 145835..147547 (-) 1713 WP_042795748.1 2-isopropylmalate synthase -
  E2544_RS00690 (E2544_00690) - 148057..148686 (-) 630 WP_042795747.1 hypothetical protein -
  E2544_RS00695 (E2544_00695) rpoN/rpoN1 148685..150130 (+) 1446 WP_063961223.1 RNA polymerase factor sigma-54 Machinery gene
  E2544_RS00700 (E2544_00700) - 150264..150545 (+) 282 WP_042795745.1 bacterioferritin-associated ferredoxin -
  E2544_RS00705 (E2544_00705) - 150576..151433 (-) 858 WP_042795744.1 LysR substrate-binding domain-containing protein -
  E2544_RS00710 (E2544_00710) - 151564..152478 (+) 915 WP_149063461.1 DMT family transporter -
  E2544_RS00715 (E2544_00715) bfr 152854..153330 (+) 477 WP_042795741.1 bacterioferritin -
  E2544_RS00720 (E2544_00720) - 153407..154090 (-) 684 WP_170946311.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52411.65 Da        Isoelectric Point: 4.8020

>NTDB_id=351667 E2544_RS00695 WP_063961223.1 148685..150130(+) (rpoN/rpoN1) [Achromobacter insolitus strain LCu2]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLERQDEAPSAEAPAESDRESSVQDDEP
AAERETPVDEMPGSGGVYPDEDSSRPEAARPDTLREHLLGQLGLTRAAPRDVALAGLLIDELDENGYLGSPLEEILDWLP
AELEPDLDELKAALSLLQSFDPPGIGARDMAECLLLQLRNPDLEQLPEAADAAVLACARQICAQHLALLATGNAARLCAA
VGCDEATFRSAHALIRRLEPRPGRAWTVPAADYAVPDVIVRKTRGGWQVTLNSAAVPKLQINGLYAQMLGNQKESAHAGL
QSQLQQARWMIRNVEQRFDTILRVAQAIVQHQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTDGGDATSATAIQTFIRQMVAEENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=351667 E2544_RS00695 WP_063961223.1 148685..150130(+) (rpoN/rpoN1) [Achromobacter insolitus strain LCu2]
ATGCAAAATATTGTACCTATGACCCGCCCAATTTTAGAACTGCGACCTGGTCAGCATTTGACGCTGACGCCACAACTGCA
GCAATCGATCCGATTGTTGCAGCTGTCCACGCTCGACCTCGAAGCCGAGATCTCGCAAGTGCTGGCGGAGAACCCGCTGC
TGGAGCGCCAGGACGAAGCGCCGTCGGCCGAGGCGCCAGCGGAATCCGACCGCGAATCCTCCGTGCAGGACGACGAACCC
GCCGCTGAGCGCGAGACGCCAGTGGATGAGATGCCGGGGTCCGGCGGCGTCTATCCCGACGAGGACTCCAGCCGGCCCGA
GGCCGCGCGCCCTGACACGCTGCGCGAGCATCTGCTGGGCCAACTGGGGCTGACGCGGGCCGCCCCGAGGGACGTCGCGC
TGGCCGGCCTCCTGATCGACGAACTGGACGAGAACGGGTACCTGGGATCGCCGCTGGAGGAAATCCTGGACTGGTTGCCG
GCGGAACTGGAGCCGGACCTGGATGAGCTGAAGGCGGCCCTGTCGCTGCTCCAGTCCTTCGATCCCCCAGGCATCGGCGC
GCGCGACATGGCGGAATGCCTGCTGTTGCAGCTGCGCAACCCGGACCTGGAACAACTGCCTGAAGCGGCGGATGCCGCGG
TATTGGCCTGTGCACGCCAGATCTGCGCGCAGCACCTGGCCCTGTTGGCGACGGGCAACGCCGCGCGCCTGTGCGCCGCC
GTGGGTTGCGATGAAGCCACGTTCCGTTCGGCCCATGCGCTCATCCGGCGCCTGGAGCCGCGTCCGGGGCGCGCCTGGAC
CGTGCCCGCGGCCGACTACGCCGTGCCCGACGTCATCGTGCGCAAGACCCGCGGCGGCTGGCAGGTGACCCTGAACAGCG
CCGCCGTGCCCAAGCTGCAGATCAACGGGCTCTACGCCCAGATGCTGGGGAACCAAAAGGAATCCGCGCACGCGGGGCTG
CAATCGCAGCTGCAGCAGGCACGCTGGATGATCCGCAATGTGGAGCAGCGTTTCGACACCATCCTGCGCGTAGCCCAGGC
CATCGTGCAGCACCAGACGGCTTTTTTCAGCCAGGGGCCTGCCGCCATGCGGCCGCTCATCCTGAAGGACATCGCTGGCG
AGCTCGGTTTGCACGAATCCACGATTTCACGGGCCACAACACAGAAGTACATGCTCACTCCGTTTGGCACGTTGGAGCTC
AAGCGCTTCTTTGGCACGGGCGTGTCGACTGACGGCGGGGACGCCACCTCGGCCACGGCGATACAGACCTTCATCCGGCA
GATGGTGGCGGAGGAGAACCGGGCCAAGCCTTTGTCCGATAGCCAGATCATGCAAAAGCTGGCCGATCAGGGGATAGTCA
TCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATAGCCCCGGCGGCATTGCGCAAGGCCCAGGCGGCCGCC
CGTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.111

100

0.503

Multiple sequence alignment