Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E3H47_RS02145 Genome accession   NZ_CP038022
Coordinates   459380..460183 (+) Length   267 a.a.
NCBI ID   WP_005016505.1    Uniprot ID   A0A2T1J2S1
Organism   Acinetobacter radioresistens strain DD78     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454380..465183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3H47_RS02120 (E3H47_02120) - 455307..456584 (+) 1278 WP_138000485.1 glycosyltransferase family 1 protein -
  E3H47_RS02125 (E3H47_02125) - 456613..457164 (+) 552 WP_005023384.1 phosphatase PAP2 family protein -
  E3H47_RS02130 (E3H47_02130) iscX 457252..457452 (+) 201 WP_005016518.1 Fe-S cluster assembly protein IscX -
  E3H47_RS02135 (E3H47_02135) ndk 457566..457997 (+) 432 WP_005016515.1 nucleoside-diphosphate kinase -
  E3H47_RS02140 (E3H47_02140) rlmN 458120..459355 (+) 1236 WP_005023387.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E3H47_RS02145 (E3H47_02145) pilF 459380..460183 (+) 804 WP_005016505.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E3H47_RS02150 (E3H47_02150) - 460174..460977 (+) 804 WP_005023389.1 helix-turn-helix domain-containing protein -
  E3H47_RS02155 (E3H47_02155) ispG 461000..462115 (+) 1116 WP_005016500.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E3H47_RS02160 (E3H47_02160) hisS 462112..463407 (+) 1296 WP_005023391.1 histidine--tRNA ligase -
  E3H47_RS02165 (E3H47_02165) - 463439..464143 (+) 705 WP_005023393.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30279.57 Da        Isoelectric Point: 9.5400

>NTDB_id=351495 E3H47_RS02145 WP_005016505.1 459380..460183(+) (pilF) [Acinetobacter radioresistens strain DD78]
MRKPVYKTFVLLTVTLSALLVTACQTPDTLSKDPEKAVKVRTQLAAEYIKSGDLDAAKRALDQALEVDSRDATANMMMGV
LLQQEGSQLNLDKAESYFRRSISIEPKNAQARNNYGAYLYQIGRYKDAIEQLEIAGATLGYEQRYQALENLGRIYLKLGN
VANAEKTFKQALQANRDSSISMLELAEIFYLRQQIPAATQLYEQYVRRVGQKNQGARALWIGIRIARANDDKMGTQVLVN
QLRALFPDSQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=351495 E3H47_RS02145 WP_005016505.1 459380..460183(+) (pilF) [Acinetobacter radioresistens strain DD78]
TTGAGAAAGCCGGTGTATAAAACATTTGTATTACTTACAGTGACTTTGTCGGCCTTGCTTGTTACTGCTTGCCAGACTCC
TGATACCCTCAGTAAGGATCCAGAAAAGGCAGTCAAGGTTCGAACCCAGCTGGCAGCGGAATATATTAAATCGGGTGATC
TGGATGCTGCTAAACGGGCACTAGACCAGGCACTTGAAGTTGATTCCAGAGACGCCACTGCGAACATGATGATGGGAGTA
CTGCTCCAGCAAGAGGGGAGTCAGCTAAATCTGGACAAGGCTGAGAGTTATTTCAGACGCTCTATTTCAATTGAGCCTAA
AAATGCACAGGCGCGTAATAATTATGGCGCCTATTTGTACCAGATCGGTCGTTATAAAGATGCGATCGAACAACTGGAAA
TTGCTGGTGCAACTTTAGGCTATGAGCAGCGTTACCAGGCGCTTGAAAATCTGGGACGAATTTATCTTAAGCTCGGCAAT
GTTGCGAATGCTGAAAAAACATTCAAACAGGCATTGCAAGCTAACCGCGATTCTTCCATTTCAATGTTAGAGTTAGCGGA
AATTTTCTATTTGCGGCAACAGATTCCTGCTGCTACACAGCTTTATGAACAATATGTGCGTCGTGTAGGGCAAAAGAATC
AGGGTGCGCGTGCTCTTTGGATCGGAATTCGAATTGCGCGTGCCAATGATGATAAAATGGGAACACAAGTTTTGGTGAAC
CAGTTACGTGCCTTGTTCCCGGACAGCCAAGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(44-82)

Tetratricopeptide repeat (TPR_16)

(150-208)

Tetratricopeptide repeat (TPR_17)

(98-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T1J2S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.794
  pilF Acinetobacter baylyi ADP1

73.81

94.382

0.697

Multiple sequence alignment