Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E2636_RS00640 Genome accession   NZ_CP038015
Coordinates   131194..132570 (+) Length   458 a.a.
NCBI ID   WP_134208105.1    Uniprot ID   A0A4P6ZWA3
Organism   Paenisporosarcina antarctica strain CGMCC 1.6503     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 126194..137570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2636_RS00620 (E2636_00620) - 126578..127048 (+) 471 WP_134208097.1 CtsR family transcriptional regulator -
  E2636_RS00625 (E2636_00625) - 127060..127602 (+) 543 WP_134208099.1 UvrB/UvrC motif-containing protein -
  E2636_RS00630 (E2636_00630) - 127592..128692 (+) 1101 WP_134208101.1 protein arginine kinase -
  E2636_RS00635 (E2636_00635) - 128682..131132 (+) 2451 WP_134208103.1 ATP-dependent Clp protease ATP-binding subunit -
  E2636_RS00640 (E2636_00640) radA 131194..132570 (+) 1377 WP_134208105.1 DNA repair protein RadA Machinery gene
  E2636_RS00645 (E2636_00645) - 132738..133817 (+) 1080 WP_134208108.1 PIN/TRAM domain-containing protein -
  E2636_RS00650 (E2636_00650) ispD 133902..134597 (+) 696 WP_134208110.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  E2636_RS00655 (E2636_00655) ispF 134598..135074 (+) 477 WP_134208112.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E2636_RS00660 (E2636_00660) gltX 135131..136594 (+) 1464 WP_134208114.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50336.89 Da        Isoelectric Point: 6.4051

>NTDB_id=351300 E2636_RS00640 WP_134208105.1 131194..132570(+) (radA) [Paenisporosarcina antarctica strain CGMCC 1.6503]
MAKRKTKFMCNSCGYESAKWMGRCPGCNEWNTMVEEVEVVVKGPRRTFQHSENSSQKALPITAIETDDEPRVKIDLSEFD
RVLGGGIVPGSLILIGGDPGIGKSTLLLQVSALLANKGQRVLYMSGEESIRQTKLRAERLGVDSSELYIYAETNLELVNE
AIDQVEPKFVVIDSIQTVHHPDVTSAPGSVTQVRECTAELMRIAKTKNIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHTYRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTVVASMEGTRPILVEIQALITPTSFN
YPKRMASGIDQNRVQLLMAVLEKRVGLLLQTQDAYIKVAGGVKLDEPAIDLAVLTTIVSSYKDEAVSAYDCFMGEVGLTG
EVRRVSRVEQRVLEAAKLGFKRAIVPASNLGGWDYPKGIQVVGVENVSDALKIAFPNT

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=351300 E2636_RS00640 WP_134208105.1 131194..132570(+) (radA) [Paenisporosarcina antarctica strain CGMCC 1.6503]
ATGGCTAAACGTAAAACGAAATTTATGTGTAACTCATGTGGCTATGAATCCGCGAAATGGATGGGTCGCTGCCCTGGTTG
CAACGAGTGGAATACGATGGTGGAAGAGGTAGAAGTAGTCGTAAAAGGACCTCGTCGGACTTTCCAACATTCTGAAAATA
GTTCACAAAAAGCTTTGCCTATTACAGCGATAGAAACGGACGACGAACCACGTGTGAAAATTGATCTTTCTGAGTTTGAT
CGTGTACTCGGTGGAGGCATCGTGCCTGGTTCACTAATATTAATTGGTGGAGATCCAGGTATTGGGAAATCCACGTTATT
GCTTCAAGTTTCCGCGTTACTTGCCAATAAAGGGCAGCGCGTGTTGTACATGTCTGGGGAGGAGTCTATTCGCCAAACGA
AGCTTCGTGCAGAGCGACTTGGTGTTGACTCTTCTGAATTATATATATATGCCGAAACTAACTTGGAATTGGTAAACGAA
GCAATTGATCAAGTTGAACCGAAGTTTGTCGTTATCGATTCTATCCAAACGGTGCATCATCCAGATGTCACTTCTGCCCC
AGGTAGCGTTACTCAGGTGCGCGAATGTACAGCGGAGTTAATGCGCATCGCTAAAACGAAAAATATTGCTATTTTCTTAG
TGGGACACGTAACAAAAGAAGGTCAAATTGCAGGGCCGAGAATACTTGAACATATGGTTGACACAGTCCTTTACTTCGAA
GGTGAACGTCACCATACATACCGTATTTTGCGTTCGCAGAAAAACCGTTTTGGCTCAACAAATGAAATTGCAATTTTTGA
AATGTTACAAGGTGGCTTAAAAGAAGTGCTAAATCCGTCTGAGTTATTCTTGCAGGAACGTTCACAAGGAGCAGCAGGGT
CCACTGTTGTAGCTTCAATGGAAGGCACCCGACCGATACTGGTCGAAATTCAGGCATTAATTACACCGACTAGTTTTAAC
TACCCAAAACGGATGGCAAGTGGCATTGATCAAAATCGTGTCCAACTATTAATGGCTGTATTGGAGAAGCGAGTTGGCCT
ATTGTTGCAAACGCAAGATGCCTACATCAAAGTTGCAGGTGGCGTAAAGTTAGACGAGCCAGCCATTGATTTGGCTGTAT
TAACGACAATTGTTTCAAGTTACAAAGATGAAGCAGTAAGTGCCTATGATTGTTTTATGGGTGAAGTGGGATTAACGGGC
GAAGTGCGTCGCGTGTCACGAGTTGAACAACGTGTGCTAGAAGCAGCGAAACTTGGTTTCAAACGTGCAATTGTTCCTGC
ATCCAACCTAGGTGGATGGGACTATCCAAAAGGTATTCAAGTAGTCGGTGTCGAAAACGTAAGCGATGCATTAAAAATAG
CTTTCCCAAACACGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(82-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P6ZWA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.524

100

0.705
  radA Streptococcus pneumoniae Rx1

61.842

99.563

0.616
  radA Streptococcus pneumoniae D39

61.842

99.563

0.616
  radA Streptococcus pneumoniae R6

61.842

99.563

0.616
  radA Streptococcus pneumoniae TIGR4

61.842

99.563

0.616
  radA Streptococcus mitis NCTC 12261

61.842

99.563

0.616
  radA Streptococcus mitis SK321

61.623

99.563

0.614

Multiple sequence alignment