Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E2C16_RS14110 Genome accession   NZ_CP038012
Coordinates   2944730..2946103 (+) Length   457 a.a.
NCBI ID   WP_115363687.1    Uniprot ID   A0A380CL08
Organism   Sporosarcina pasteurii strain BNCC 337394     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2939730..2951103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2C16_RS14090 (E2C16_14090) - 2939947..2940414 (+) 468 WP_115363695.1 CtsR family transcriptional regulator -
  E2C16_RS14095 (E2C16_14095) - 2940452..2941006 (+) 555 WP_115363693.1 UvrB/UvrC motif-containing protein -
  E2C16_RS14100 (E2C16_14100) - 2940996..2942087 (+) 1092 WP_115363691.1 protein arginine kinase -
  E2C16_RS14105 (E2C16_14105) - 2942211..2944652 (+) 2442 WP_115363690.1 ATP-dependent Clp protease ATP-binding subunit -
  E2C16_RS14110 (E2C16_14110) radA 2944730..2946103 (+) 1374 WP_115363687.1 DNA repair protein RadA Machinery gene
  E2C16_RS14115 (E2C16_14115) - 2946237..2947343 (+) 1107 WP_115363685.1 PIN/TRAM domain-containing protein -
  E2C16_RS14120 (E2C16_14120) ispD 2947377..2948072 (+) 696 WP_115363683.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  E2C16_RS14125 (E2C16_14125) ispF 2948069..2948545 (+) 477 WP_115363681.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E2C16_RS14130 (E2C16_14130) gltX 2949093..2950553 (+) 1461 WP_115363679.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50353.80 Da        Isoelectric Point: 6.1987

>NTDB_id=351295 E2C16_RS14110 WP_115363687.1 2944730..2946103(+) (radA) [Sporosarcina pasteurii strain BNCC 337394]
MSKRKTKFMCKDCGYESLRWMGRCPGCGEWNTMNEEVEVVRKGPRGAFQHTDEVQQKAVPISQVETIEEPRVETELGELN
RVLGGGIVPGSLLLTAGDPGIGKSTLLLQVSSLLANQNHRVLYISGEESIRQTKLRAERLGVLSDELFIYAETDLERIHH
TIDEINPAFVIVDSIQTIHHPEVKSAPGSITQVRECTAELMRIAKTKNIAIFLVGHVTKDGQIAGPRILEHMVDTVLYFE
GERHHTYRILRSVKNRFGSTNEIAIFEMLQSGLKEVLNPSELFLRERSQGGAGSTVVASMEGTRPILVEIQALVTPSSFN
YPKRMATGLDQNRVSLLMAVLEKRMGMLLQAQDAYIKVAGGVKLDEPAIDLAVLVSIVSSYRDSAAGLSDCFIGEVGLTG
EVRRVSRIEQRVQEAAKLGFERAIIPSSNLGGWDYPEGIQVIGVETVSDALKVGFSQ

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=351295 E2C16_RS14110 WP_115363687.1 2944730..2946103(+) (radA) [Sporosarcina pasteurii strain BNCC 337394]
ATGTCAAAGAGAAAAACAAAATTTATGTGTAAAGATTGTGGGTACGAGTCTCTTCGGTGGATGGGACGTTGCCCGGGTTG
CGGGGAATGGAACACGATGAACGAGGAAGTGGAAGTGGTTCGAAAAGGTCCACGTGGTGCGTTCCAACATACCGATGAAG
TTCAACAAAAAGCAGTGCCGATTAGTCAAGTTGAGACGATTGAGGAACCGCGTGTTGAAACAGAATTAGGAGAGTTAAAC
CGAGTGCTAGGCGGTGGCATTGTGCCAGGTTCACTGTTGTTAACTGCAGGAGACCCCGGAATTGGAAAATCTACTTTGCT
CCTTCAAGTTTCCTCGCTTCTCGCCAACCAGAATCATCGTGTATTGTATATATCTGGAGAAGAATCCATTCGACAAACAA
AGCTTCGCGCGGAAAGGTTGGGGGTTCTTTCGGACGAATTGTTTATTTATGCGGAAACGGATCTAGAAAGAATTCATCAC
ACAATCGATGAAATTAACCCTGCGTTTGTGATTGTAGATTCTATACAAACCATCCATCATCCTGAAGTCAAGTCCGCACC
GGGAAGTATTACACAAGTAAGAGAGTGTACGGCGGAATTAATGCGTATCGCGAAAACCAAAAACATTGCCATATTCTTAG
TGGGTCACGTAACAAAAGATGGACAGATTGCTGGACCACGTATTTTGGAACATATGGTAGATACAGTTTTATACTTTGAA
GGGGAACGTCATCATACATATCGTATTTTACGAAGTGTGAAAAATAGATTTGGTTCAACGAATGAGATCGCTATTTTTGA
AATGTTGCAATCGGGTCTAAAGGAAGTTTTAAATCCATCCGAGTTGTTTTTAAGAGAGCGTTCTCAAGGTGGTGCCGGTT
CAACCGTCGTCGCATCTATGGAAGGTACGAGGCCGATTCTCGTAGAAATCCAAGCACTTGTGACGCCTTCAAGCTTTAAC
TATCCAAAGCGGATGGCGACAGGATTGGATCAAAACCGGGTTTCGCTATTAATGGCCGTGCTTGAAAAACGAATGGGCAT
GCTGTTACAAGCACAAGATGCTTATATTAAAGTTGCTGGTGGTGTGAAATTAGACGAACCTGCTATCGATTTAGCCGTCC
TAGTTAGTATCGTCTCTAGTTATCGAGATTCTGCCGCTGGATTATCAGATTGCTTTATCGGAGAGGTTGGTCTAACGGGA
GAGGTGCGCCGTGTTTCTAGAATTGAACAACGTGTGCAAGAGGCAGCGAAGCTTGGATTCGAGCGGGCAATTATTCCGTC
TTCCAATTTAGGTGGATGGGACTATCCGGAAGGGATTCAAGTGATCGGTGTTGAAACAGTTAGTGATGCGTTAAAAGTTG
GGTTCTCGCAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(359-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380CL08

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.996

100

0.691
  radA Streptococcus pneumoniae Rx1

61.269

100

0.613
  radA Streptococcus pneumoniae D39

61.269

100

0.613
  radA Streptococcus pneumoniae R6

61.269

100

0.613
  radA Streptococcus pneumoniae TIGR4

61.269

100

0.613
  radA Streptococcus mitis NCTC 12261

61.269

100

0.613
  radA Streptococcus mitis SK321

61.269

100

0.613

Multiple sequence alignment