Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E2K99_RS20675 Genome accession   NZ_CP037993
Coordinates   4632510..4634000 (+) Length   496 a.a.
NCBI ID   WP_134223406.1    Uniprot ID   -
Organism   Herbaspirillum huttiense strain NFYY 53159     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4627510..4639000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K99_RS20645 (E2K99_20645) - 4628432..4628758 (+) 327 WP_134223403.1 hypothetical protein -
  E2K99_RS20650 (E2K99_20650) - 4628755..4629783 (+) 1029 WP_039784050.1 KpsF/GutQ family sugar-phosphate isomerase -
  E2K99_RS20655 (E2K99_20655) - 4629783..4630337 (+) 555 WP_034331440.1 KdsC family phosphatase -
  E2K99_RS20660 (E2K99_20660) lptC 4630334..4630942 (+) 609 WP_134223404.1 LPS export ABC transporter periplasmic protein LptC -
  E2K99_RS20665 (E2K99_20665) lptA 4630994..4631632 (+) 639 WP_034331445.1 lipopolysaccharide transport periplasmic protein LptA -
  E2K99_RS20670 (E2K99_20670) lptB 4631635..4632378 (+) 744 WP_134223405.1 LPS export ABC transporter ATP-binding protein -
  E2K99_RS20675 (E2K99_20675) rpoN/rpoN1 4632510..4634000 (+) 1491 WP_134223406.1 RNA polymerase factor sigma-54 Machinery gene
  E2K99_RS20680 (E2K99_20680) hpf 4634153..4634515 (+) 363 WP_034331451.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E2K99_RS25610 - 4634578..4634724 (-) 147 WP_157845661.1 hypothetical protein -
  E2K99_RS20685 (E2K99_20685) grxD 4634886..4635197 (+) 312 WP_039784040.1 Grx4 family monothiol glutaredoxin -
  E2K99_RS20690 (E2K99_20690) - 4635220..4635822 (+) 603 WP_034331455.1 UbiX family flavin prenyltransferase -
  E2K99_RS20695 (E2K99_20695) - 4635911..4636669 (-) 759 WP_039784036.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55117.63 Da        Isoelectric Point: 5.1963

>NTDB_id=351151 E2K99_RS20675 WP_134223406.1 4632510..4634000(+) (rpoN/rpoN1) [Herbaspirillum huttiense strain NFYY 53159]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDNPLLERLDDALDNSVRLLADGAVSSSPSSGVAETSHE
GSGEAAPAASDGDSSFDYQDNAPEAGVTGDADWSFDDVARSGKSPDDDDARPQLEAHELTLREHLLEQIRVTARTLRDRA
LLELLTDALDETGYLEEPLEDIHARLPEELGIDMEELSISLKLLQSLDPAGVGARSAGECLALQIRRLPKIPFVTRKLAL
RIVEDYLPLFAQRDFNKIKKALDCDDEDLREAQAVIRQCRPHPGAEFARDASDYVVPDVIVKQTKNGWQVMLNHEVMPKL
RVNAMYASALKQAKGEGSLSSQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHEST
ISRVTTQKYMLTPHGMFELKYFFGSHVATETGGEASSTAIRALIKQLIGAENPQTPLSDSKIADMLAEQGMVVARRTVAK
YREVLKIPPVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=351151 E2K99_RS20675 WP_134223406.1 4632510..4634000(+) (rpoN/rpoN1) [Herbaspirillum huttiense strain NFYY 53159]
ATGAAACAGTCGCTCCAGTTACGTACCTCGCAGCACCTCGCATTGACGCCGCAACTGCAGCAGTCGATCAGACTGTTGCA
ACTGTCGACGCTGGAACTGCACCAGGAACTGGAACAGATCCTCTCCGACAATCCCCTGCTGGAACGCCTCGACGACGCGC
TCGACAATTCGGTACGTCTGCTGGCCGATGGCGCGGTCTCCAGTTCGCCCTCCAGCGGCGTTGCGGAAACCAGTCACGAA
GGCAGTGGCGAAGCGGCCCCGGCCGCCAGCGATGGCGATTCCAGCTTCGACTACCAGGACAACGCGCCGGAAGCCGGTGT
CACGGGGGATGCCGACTGGAGCTTCGACGACGTCGCGCGCAGTGGCAAGTCGCCAGACGATGACGATGCCCGTCCGCAAC
TCGAAGCGCACGAACTGACCCTGCGCGAACACCTGCTGGAACAGATCCGCGTGACCGCACGCACCCTGCGTGATCGTGCG
CTGCTGGAACTGCTCACCGATGCGCTCGACGAGACCGGCTATCTGGAAGAGCCGCTGGAAGACATCCACGCACGCCTGCC
CGAAGAGCTGGGCATCGACATGGAAGAGCTGTCGATTTCGCTCAAGCTGCTGCAGAGCCTGGACCCGGCGGGTGTCGGTG
CGCGCAGTGCCGGCGAATGCCTGGCCCTGCAGATCCGCCGCCTGCCCAAGATTCCCTTCGTGACGCGCAAGCTGGCGCTG
AGGATCGTGGAGGATTACCTGCCGCTGTTTGCACAACGCGATTTCAACAAGATCAAGAAGGCACTGGATTGCGATGACGA
AGACCTGCGTGAAGCGCAGGCCGTGATCCGCCAGTGCCGTCCGCATCCGGGTGCGGAATTCGCGCGTGATGCCTCGGACT
ATGTGGTGCCGGACGTGATTGTCAAGCAGACAAAGAACGGCTGGCAGGTCATGCTCAACCATGAGGTCATGCCCAAGCTG
CGCGTCAATGCGATGTATGCCAGTGCATTGAAGCAAGCCAAGGGAGAAGGTTCGCTGTCGTCGCAGTTGCAGGAAGCCAA
GTGGCTCATCAAGAACATGCGGCAACGTTTCGATACAATTTTGCGCGTCGCGCAGGCTATTGTGGAACGTCAAAGAAACT
TCTTTTCACATGGGGCAGTGGCCATGCGCCCCCTTGTGCTGCGTGAAATTGCTGATACACTGGGTCTACACGAGAGCACC
ATCTCTCGCGTGACGACTCAGAAATACATGCTCACTCCGCATGGAATGTTTGAGTTGAAATACTTCTTCGGTAGCCATGT
CGCGACTGAAACCGGAGGTGAAGCATCCTCCACCGCGATACGGGCGCTGATCAAACAGTTGATAGGAGCCGAGAACCCGC
AGACCCCATTATCCGATAGCAAGATTGCGGATATGCTGGCAGAACAAGGCATGGTGGTCGCGCGACGCACAGTCGCCAAA
TACCGCGAAGTGTTGAAGATTCCGCCGGTCAATCTCCGCAAGTCCCTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.929

100

0.599

Multiple sequence alignment