Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E2K99_RS11240 Genome accession   NZ_CP037993
Coordinates   2468855..2470240 (+) Length   461 a.a.
NCBI ID   WP_134222252.1    Uniprot ID   A0AAE4G5S1
Organism   Herbaspirillum huttiense strain NFYY 53159     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2463855..2475240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2K99_RS11220 (E2K99_11220) - 2464075..2465094 (+) 1020 WP_134222249.1 uracil-DNA glycosylase -
  E2K99_RS11225 (E2K99_11225) - 2465108..2466118 (+) 1011 WP_134222250.1 DUF1853 family protein -
  E2K99_RS11230 (E2K99_11230) lplT 2466152..2467423 (-) 1272 WP_121039922.1 lysophospholipid transporter LplT -
  E2K99_RS11235 (E2K99_11235) alr 2467692..2468771 (+) 1080 WP_134222251.1 alanine racemase -
  E2K99_RS11240 (E2K99_11240) radA 2468855..2470240 (+) 1386 WP_134222252.1 DNA repair protein RadA Machinery gene
  E2K99_RS11245 (E2K99_11245) - 2470255..2470824 (-) 570 WP_134222253.1 flagellar protein FlhE -
  E2K99_RS11250 (E2K99_11250) - 2470817..2472178 (-) 1362 WP_205703774.1 sensor histidine kinase -
  E2K99_RS11255 (E2K99_11255) ompR 2472216..2472974 (-) 759 WP_134222255.1 two-component system response regulator OmpR -
  E2K99_RS11260 (E2K99_11260) - 2473164..2473445 (+) 282 WP_039788314.1 hypothetical protein -
  E2K99_RS11265 (E2K99_11265) - 2473543..2474202 (-) 660 WP_199900340.1 amino acid ABC transporter permease -
  E2K99_RS11270 (E2K99_11270) - 2474298..2475101 (-) 804 WP_134222256.1 cystine ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48920.33 Da        Isoelectric Point: 7.1306

>NTDB_id=351143 E2K99_RS11240 WP_134222252.1 2468855..2470240(+) (radA) [Herbaspirillum huttiense strain NFYY 53159]
MAKVKTNYTCTECGGVSNKWAGQCPACGQWNTLVETLVDTGGGNRYSSTPQSLAQTAPVLSLADIEAIDVPRFGTGIEEF
DRVLGGGLVPGGVALIGGDPGIGKSTLLLQALANISRLKKVLYVSGEESGSQIALRAKRLAVDARELQLQAEIQLEKILA
TLAEHKPEVAVIDSIQTIYSDALTSAPGSVAQVRECAAQLTRVAKTSGITIIMVGHVTKEGALAGPRVLEHIVDTVLYFE
GDTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLKGVSNPSALFLSQHENQVPGSCVMVTQEGTRPLLVEIQALVDSSHVP
NARRLSVGLEQNRLAMLLAVLHRHAGVAAFDQDVFINAVGGVKITEPAADLAVLLAINSSMRNKPLPRGLVVFGEVGLAG
EIRPAPRGQERLREAAKLGFSIAVIPKANAPKQAIEGLKVIPVERIDDALQKAREIDDYAA

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=351143 E2K99_RS11240 WP_134222252.1 2468855..2470240(+) (radA) [Herbaspirillum huttiense strain NFYY 53159]
ATGGCGAAGGTCAAGACCAATTACACCTGTACCGAATGCGGCGGGGTCAGCAACAAATGGGCCGGGCAGTGTCCGGCCTG
CGGGCAGTGGAATACCTTGGTCGAGACGCTGGTGGACACCGGTGGCGGGAATCGCTATTCGTCGACCCCGCAAAGCCTGG
CGCAGACCGCACCGGTACTGAGCCTGGCCGATATCGAAGCGATCGACGTGCCGCGCTTTGGCACTGGGATCGAGGAATTC
GACCGCGTGCTGGGCGGTGGCCTGGTGCCGGGCGGAGTGGCGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCGACCTT
GCTGCTGCAGGCACTGGCCAATATCTCCCGGCTCAAGAAGGTACTCTATGTGAGCGGCGAAGAGTCCGGTTCGCAGATCG
CCTTGCGCGCCAAGCGCCTGGCGGTGGATGCCCGTGAACTGCAATTGCAGGCCGAGATCCAGCTGGAAAAGATCCTCGCC
ACGCTGGCCGAGCACAAGCCCGAAGTGGCCGTGATCGATTCCATCCAGACCATTTATTCCGATGCGCTGACCTCGGCCCC
CGGCTCGGTGGCGCAGGTGCGCGAATGCGCGGCGCAACTGACGCGGGTGGCCAAGACTTCCGGCATCACCATCATCATGG
TCGGCCACGTGACCAAGGAAGGTGCGCTGGCCGGTCCGCGCGTGCTGGAACACATCGTCGATACGGTGCTGTACTTCGAG
GGCGATACGCATTCCAGCTTCCGCCTGGTGCGCGCCTTCAAGAACCGCTTCGGCGCAGTGAACGAACTGGGCGTGTTTGC
CATGACCGAGAAGGGCCTGAAGGGGGTCTCCAATCCTTCTGCGCTGTTCCTTTCGCAGCACGAAAACCAGGTCCCCGGCT
CCTGCGTCATGGTGACCCAGGAAGGCACGCGTCCGCTGCTGGTGGAAATCCAGGCCTTGGTGGACAGCTCGCACGTTCCC
AACGCGCGCCGCCTGTCGGTCGGTCTGGAGCAGAACCGCCTGGCCATGCTGCTGGCGGTGCTGCACCGCCACGCGGGGGT
GGCGGCTTTCGACCAAGATGTCTTCATCAATGCGGTCGGCGGGGTGAAGATTACCGAACCGGCGGCCGACCTGGCGGTGC
TGCTGGCGATCAATTCCTCCATGCGCAACAAGCCCTTGCCGCGCGGATTGGTGGTGTTCGGTGAAGTGGGTCTGGCTGGG
GAGATCCGCCCAGCGCCGCGCGGCCAGGAGCGGCTGCGCGAGGCCGCCAAGCTGGGCTTCTCGATTGCGGTCATTCCCAA
GGCCAATGCACCCAAGCAGGCTATTGAGGGATTGAAGGTGATTCCGGTGGAGCGCATCGACGACGCTCTGCAGAAGGCGC
GCGAGATCGACGATTACGCTGCCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(360-453)

AAA domain (AAA_25)

(77-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.701

100

0.488
  radA Streptococcus mitis SK321

48.584

99.566

0.484
  radA Streptococcus pneumoniae D39

48.69

99.349

0.484
  radA Streptococcus pneumoniae TIGR4

48.69

99.349

0.484
  radA Streptococcus pneumoniae R6

48.69

99.349

0.484
  radA Streptococcus pneumoniae Rx1

48.69

99.349

0.484
  radA Streptococcus mitis NCTC 12261

48.472

99.349

0.482

Multiple sequence alignment