Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E2R28_RS18690 Genome accession   NZ_CP037974
Coordinates   532709..534028 (-) Length   439 a.a.
NCBI ID   WP_038737839.1    Uniprot ID   -
Organism   Burkholderia pseudomallei strain BPs114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527709..539028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2R28_RS18670 (E2R28_18675) - 528608..529003 (-) 396 WP_004530943.1 nitrite reductase (NAD(P)H) small subunit -
  E2R28_RS18675 (E2R28_18680) nirB 529079..531523 (-) 2445 WP_134162300.1 nitrite reductase large subunit NirB -
  E2R28_RS18680 (E2R28_18685) - 531570..531911 (+) 342 WP_009918580.1 hypothetical protein -
  E2R28_RS18685 (E2R28_18690) maiA 531908..532552 (+) 645 WP_131117436.1 maleylacetoacetate isomerase -
  E2R28_RS18690 (E2R28_18695) pilA 532709..534028 (-) 1320 WP_038737839.1 signal recognition particle-docking protein FtsY Machinery gene
  E2R28_RS18695 (E2R28_18700) rsmD 534280..534906 (+) 627 WP_004522861.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  E2R28_RS18705 (E2R28_18710) coaD 535017..535517 (+) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  E2R28_RS18710 (E2R28_18715) - 535580..535846 (+) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  E2R28_RS18715 (E2R28_18720) hisC 536013..537083 (-) 1071 WP_004553797.1 histidinol-phosphate transaminase -
  E2R28_RS18720 (E2R28_18725) pth 537172..537777 (-) 606 WP_004202941.1 aminoacyl-tRNA hydrolase -
  E2R28_RS18725 (E2R28_18730) - 537891..538505 (-) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46293.58 Da        Isoelectric Point: 6.1951

>NTDB_id=351111 E2R28_RS18690 WP_038737839.1 532709..534028(-) (pilA) [Burkholderia pseudomallei strain BPs114]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDEHSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLGALREKVRAGRLTDPQQVKDALRGLLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=351111 E2R28_RS18690 WP_038737839.1 532709..534028(-) (pilA) [Burkholderia pseudomallei strain BPs114]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCACA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAGCCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGATGCGCTGCGCGGGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(240-439)

SRP54-type protein, helical bundle domain (SRP54_N)

(158-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.554

100

0.506

Multiple sequence alignment