Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EW139_RS01590 Genome accession   NZ_CP037939
Coordinates   215537..216904 (+) Length   455 a.a.
NCBI ID   WP_083812261.1    Uniprot ID   -
Organism   Leuconostoc kimchii strain NKJ218     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 210537..221904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW139_RS01560 (EW139_01560) - 210845..212269 (-) 1425 WP_134833444.1 aspartate ammonia-lyase -
  EW139_RS01565 (EW139_01565) rpiA 212454..213137 (-) 684 WP_013975332.1 ribose-5-phosphate isomerase RpiA -
  EW139_RS01570 (EW139_01570) - 213134..213427 (-) 294 WP_134833445.1 GNAT family N-acetyltransferase -
  EW139_RS01575 (EW139_01575) - 213657..214196 (+) 540 WP_013103000.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  EW139_RS01580 (EW139_01580) - 214196..214669 (+) 474 WP_013975330.1 NUDIX domain-containing protein -
  EW139_RS01585 (EW139_01585) - 214674..215534 (+) 861 WP_013975329.1 metallophosphoesterase family protein -
  EW139_RS01590 (EW139_01590) radA 215537..216904 (+) 1368 WP_083812261.1 DNA repair protein RadA Machinery gene
  EW139_RS01595 (EW139_01595) - 216929..217267 (+) 339 WP_013103005.1 hypothetical protein -
  EW139_RS01600 (EW139_01600) - 217414..218454 (-) 1041 WP_013975327.1 DUF6287 domain-containing protein -
  EW139_RS01605 (EW139_01605) - 218573..219097 (-) 525 WP_013975326.1 hypothetical protein -
  EW139_RS01610 (EW139_01610) - 219337..219645 (+) 309 WP_013975325.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49094.23 Da        Isoelectric Point: 5.1552

>NTDB_id=350751 EW139_RS01590 WP_083812261.1 215537..216904(+) (radA) [Leuconostoc kimchii strain NKJ218]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIAPEVNDRKSRVSLDGRTAKVEKINEITSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IDELQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISVFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFIGEIGLTGE
VRSVADIEGRLKEARKLGFKRAIVPKNNLNGVELPAGIQVVGVTTLSEALKLALE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=350751 EW139_RS01590 WP_083812261.1 215537..216904(+) (radA) [Leuconostoc kimchii strain NKJ218]
ATCGCTAAAGTTAAAACACAATTTATTTGTTCGAACTGTGGCTTCACATCAGCACGTTACCTTGGCCGGTGTTCAAATTG
TGGTGAGTGGGGTACCTTTGTTGAAGAAAAAATTGCGCCTGAAGTTAATGATCGTAAAAGTCGTGTGAGCCTTGATGGAC
GCACGGCAAAAGTTGAGAAAATTAACGAGATTACTTCTGAAGAGACACCACGCGTGGCAACGAATCTGAAAGAACTTAAT
CGTGTTTTAGGTGGCGGTGTTGTTCCGGGATCCATGGTGTTGATTGGTGGTGATCCTGGTATTGGAAAATCAACGCTGTT
GTTGCAGGTATCAGGGCAATTGGCACATGAGGGACGTGTTTTGTACGTTACTGGTGAAGAATCAGCCACACAAGTTAAGT
TGCGAGCTGACCGTTTGGGTGTCGGAAACGATGAATTTTACTTGTATCCAGAAACTGATATGACGGCTATTAAAAAACAG
ATTGATGAATTACAACCTGATTTTGTCGTAATCGATTCCGTGCAAACGATGCAAGAGCCAGATGTCACATCTGCTATTGG
ATCAGTATCACAAATTCGTGAAGTGACGGCAGATCTTTTACAAATTGCTAAAACAAATAATATTTCTGTCTTTATTGTCG
GACATGTGACCAAAGATGGTGCGATTGCAGGGCCTAAAATTTTGGAACACATGGTTGATACGGTACTTTATTTTGAGGGC
GATAGTAATTATAAATATCGTATCTTACGTGCGGTGAAAAATCGATTTGGCGCAACCAACGAATTGGGTATCTTTGAAAT
GCGTGATGGTGGCCTAATTGAAGTAGCGAACCCCTCTGAAATTTTCTTAGAGGAGCGGTTGGCTGGTGCAACTGGTTCAG
CAATTGTTGTCGCACTTGAAGGCTCACGACCGATTTTGGTGGAGTTGCAAGCTTTGGTGACACCAACCGTGTTTGGGAAT
GCACAACGAACGGCTTCGGGCTTAGATCGTAATCGTGTCTCGTTGATTATGGCGGTGCTGGAAAAGCGCGCGAATTTGTT
GTTACAAAATCAAGATGCTTATTTAAAAGCAGCGGGCGGTGTTAAATTAGATGAACCAGCGATTGATTTAGCAATTGCTG
TCGCGTTAGCTAGTTCATATCATGACAAAGAATCACGTCCGAGCGATGTGTTTATCGGCGAAATAGGGTTAACCGGTGAA
GTACGAAGCGTTGCTGATATTGAGGGACGATTAAAAGAAGCGCGTAAACTCGGATTTAAGCGTGCTATTGTGCCTAAGAA
CAATTTAAATGGTGTCGAATTACCCGCAGGTATTCAAGTTGTTGGGGTGACAACCTTGAGTGAAGCTTTGAAATTGGCTT
TGGAGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

65.854

99.121

0.653
  radA Streptococcus pneumoniae D39

65.854

99.121

0.653
  radA Streptococcus pneumoniae R6

65.854

99.121

0.653
  radA Streptococcus pneumoniae TIGR4

65.854

99.121

0.653
  radA Streptococcus mitis NCTC 12261

65.632

99.121

0.651
  radA Streptococcus mitis SK321

65.632

99.121

0.651
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624

Multiple sequence alignment