Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   GKC13_RS00875 Genome accession   NZ_CP046134
Coordinates   155450..155743 (+) Length   97 a.a.
NCBI ID   WP_011226625.1    Uniprot ID   Q5M2G2
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 150450..160743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS00850 (GKC13_00845) - 152401..152763 (+) 363 WP_011226630.1 DUF1033 family protein -
  GKC13_RS00855 (GKC13_00850) comGA/cglA/cilD 152844..153785 (+) 942 WP_071417491.1 competence type IV pilus ATPase ComGA Machinery gene
  GKC13_RS00860 (GKC13_00855) comGB 153667..154767 (+) 1101 WP_207560111.1 competence type IV pilus assembly protein ComGB Machinery gene
  GKC13_RS00865 (GKC13_00860) comGC 154764..155090 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  GKC13_RS00870 (GKC13_00865) comGD 155050..155478 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  GKC13_RS00875 (GKC13_00870) comGE 155450..155743 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  GKC13_RS00880 (GKC13_00875) comGF 155727..156164 (+) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  GKC13_RS00885 (GKC13_00880) comGG 156142..156459 (+) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG Machinery gene
  GKC13_RS00890 (GKC13_00885) comGH 156504..157469 (+) 966 WP_208301810.1 class I SAM-dependent methyltransferase Machinery gene
  GKC13_RS00895 (GKC13_00890) - 157515..158708 (+) 1194 WP_014608740.1 acetate kinase -
  GKC13_RS00900 (GKC13_00895) - 158957..159154 (+) 198 WP_014727723.1 helix-turn-helix transcriptional regulator -
  GKC13_RS09810 (GKC13_00900) - 159397..159822 (+) 426 WP_087010196.1 CPBP family intramembrane glutamic endopeptidase -
  GKC13_RS00910 (GKC13_00905) - 159896..160339 (+) 444 WP_084829882.1 CAAX protease -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10844.74 Da        Isoelectric Point: 10.4561

>NTDB_id=350128 GKC13_RS00875 WP_011226625.1 155450..155743(+) (comGE) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MVNIKRRSLRGYILIESLVAMAVLVLVSSLILEQINTNRRLMADNLHQQEVLSVATMAVQTKQDQLTLNGIAVTVKRSQQ
GITVYESGKEIIHVSKQ

Nucleotide


Download         Length: 294 bp        

>NTDB_id=350128 GKC13_RS00875 WP_011226625.1 155450..155743(+) (comGE) [Streptococcus thermophilus strain MAG_rmk202_sterm]
GTGGTAAATATAAAAAGACGATCTCTTAGAGGTTATATTCTTATTGAAAGTCTGGTAGCTATGGCTGTTTTAGTACTTGT
CAGCAGTCTGATATTGGAACAAATTAATACCAACCGCAGACTAATGGCTGATAATCTCCATCAACAAGAGGTCTTGAGTG
TGGCAACAATGGCAGTTCAGACCAAGCAAGACCAGTTAACGCTGAATGGCATAGCGGTGACAGTTAAACGTAGCCAGCAA
GGTATCACGGTTTATGAATCGGGAAAGGAGATTATTCATGTATCTAAGCAGTAA

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Streptococcus mutans UA140

43.299

100

0.433
  comGE Streptococcus mutans UA159

43.299

100

0.433
  comGE Lactococcus lactis subsp. cremoris KW2

36.082

100

0.361