Detailed information    

experimental Experimentally validated

Overview

Name   cbpD   Type   Auxiliary factor
Locus tag   STU_RS09750 Genome accession   NC_006448
Coordinates   43821..44660 (-) Length   279 a.a.
NCBI ID   WP_011225268.1    Uniprot ID   A0A8D6XSA1
Organism   Streptococcus thermophilus LMG 18311     
Function   fratricide process; lyse non-competent cells   
Cell lysis

Function

Deletion of CbpD reduces transformation efficiency in Streptococcus thermophilus.


Genomic Context

Location: 38821..49660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STU_RS09720 (stu0033) purM 39233..40255 (+) 1023 WP_288577255.1 phosphoribosylformylglycinamidine cyclo-ligase -
  STU_RS09725 (stu0034) purN 40255..40809 (+) 555 WP_011225263.1 phosphoribosylglycinamide formyltransferase -
  STU_RS09730 (stu0035) purH 40878..42425 (+) 1548 WP_002949016.1 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase -
  STU_RS19000 - 42596..43717 (+) 1122 Protein_30 IS3 family transposase -
  STU_RS09750 (stu0039) cbpD 43821..44660 (-) 840 WP_011225268.1 CHAP domain-containing protein Auxiliary factor
  STU_RS09755 (stu0040) purD 44968..46230 (+) 1263 WP_011225269.1 phosphoribosylamine--glycine ligase -
  STU_RS09760 (stu0041) purE 46576..47064 (+) 489 WP_041828093.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  STU_RS09765 (stu0042) purK 47051..48142 (+) 1092 WP_011225271.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  STU_RS09770 (stu0043) - 48179..49090 (+) 912 Protein_35 ketopantoate reductase family protein -
  STU_RS09775 (stu0044) - 49099..49326 (+) 228 Protein_36 phosphoribosylaminoimidazole carboxylase -

Sequence

Protein


Download         Length: 279 a.a.        Molecular weight: 30110.72 Da        Isoelectric Point: 6.9050

>NTDB_id=350 STU_RS09750 WP_011225268.1 43821..44660(-) (cbpD) [Streptococcus thermophilus LMG 18311]
MKKKLLNYLILLACLATVSVSCSGSAQAAVLGDDYPFSWKYGGFGVDPWTMYWRQCTSFAAYRLSNTNGFTLPIGYGNAI
TWGNIARANGHRVDMNPAVGSIAWFSAGVNGAGHMGHVAWVAEVHGDQVTIEEYNYDAGQGPEKYHKRSFHKSQVSGYIH
FKDLESGTQNGNPTNSSIKVGDSVRFTGTFRVTSVSGNTITSQDLAGGTPTKHNIVDPSPVLEVDAQGNPTSDQYLNPGE
TFTIPGNFKVLAIDPPSDGILVQIGNLKTWVTQSVLEKI

Nucleotide


Download         Length: 840 bp        

>NTDB_id=350 STU_RS09750 WP_011225268.1 43821..44660(-) (cbpD) [Streptococcus thermophilus LMG 18311]
ATGAAGAAAAAATTACTAAATTATCTCATACTGCTAGCTTGTCTAGCGACTGTTTCGGTTTCGTGCAGCGGCTCTGCTCA
AGCAGCTGTCCTCGGAGATGACTACCCTTTTTCTTGGAAATATGGTGGCTTCGGCGTGGACCCTTGGACTATGTACTGGC
GTCAGTGTACTTCCTTTGCGGCCTACCGCCTTAGCAACACCAATGGATTCACCTTACCTATAGGTTATGGAAATGCCATC
ACTTGGGGTAATATCGCACGTGCGAATGGCCACCGTGTTGACATGAATCCTGCCGTAGGGAGCATCGCCTGGTTCTCAGC
TGGTGTTAATGGAGCTGGTCACATGGGGCATGTCGCTTGGGTTGCAGAAGTACATGGTGATCAGGTAACTATTGAAGAAT
ACAACTACGATGCGGGTCAGGGGCCTGAGAAATATCACAAACGTAGCTTCCATAAGAGTCAAGTGTCTGGATATATCCAT
TTTAAAGACCTCGAATCAGGAACTCAAAATGGAAATCCTACTAATTCTTCTATAAAAGTTGGCGATTCAGTCCGATTCAC
AGGTACCTTCCGTGTCACCTCTGTGTCAGGAAATACCATCACCAGCCAAGATTTAGCTGGAGGTACACCAACCAAACACA
ATATCGTTGACCCTAGCCCCGTTCTTGAAGTTGATGCACAAGGTAATCCAACATCAGACCAGTACCTCAATCCTGGTGAG
ACCTTTACTATTCCAGGTAACTTCAAAGTTCTTGCCATTGACCCACCTAGCGATGGCATCCTCGTCCAAATCGGAAATCT
CAAGACTTGGGTAACCCAATCTGTCCTTGAAAAGATTTAA

Domains

Predicted by InterproScan.

CHAP domain (CHAP)

(48-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cbpD Streptococcus thermophilus LMD-9

99.283

100

0.993

Multiple sequence alignment    



References

[1] Truls Johan Biørnstad et al. (2012) Deletion of the murein hydrolase CbpD reduces transformation efficiency in Streptococcus thermophilus. Microbiology (Reading, England) 158(Pt 4):877-885. [PMID: 22241050]