Detailed information    

experimental Experimentally validated

Overview

Name   cbpD   Type   Auxiliary factor
Locus tag   STER_RS00295 Genome accession   NC_008532
Coordinates   45089..45928 (-) Length   279 a.a.
NCBI ID   WP_011680595.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   fratricide process; lyse non-competent cells   
Cell lysis

Function

Deletion of CbpD reduces transformation efficiency in Streptococcus thermophilus.


Genomic Context

Location: 40089..50928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS00265 (STER_0052) purM 40500..41522 (+) 1023 WP_024704311.1 phosphoribosylformylglycinamidine cyclo-ligase -
  STER_RS00270 (STER_0053) purN 41522..42076 (+) 555 WP_011225263.1 phosphoribosylglycinamide formyltransferase -
  STER_RS00275 (STER_0054) purH 42145..43692 (+) 1548 WP_011680593.1 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase -
  STER_RS09920 - 43864..44984 (+) 1121 Protein_31 IS3 family transposase -
  STER_RS00295 (STER_0057) cbpD 45089..45928 (-) 840 WP_011680595.1 CHAP domain-containing protein Auxiliary factor
  STER_RS00300 (STER_0058) purD 46237..47499 (+) 1263 WP_011226734.1 phosphoribosylamine--glycine ligase -
  STER_RS00305 (STER_0059) purE 47845..48333 (+) 489 WP_002948169.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  STER_RS00310 (STER_0060) purK 48320..49411 (+) 1092 WP_011226736.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  STER_RS00315 (STER_0061) - 49448..50359 (+) 912 Protein_36 ketopantoate reductase family protein -
  STER_RS00320 (STER_0063) - 50368..50595 (+) 228 Protein_37 phosphoribosylaminoimidazole carboxylase -

Sequence

Protein


Download         Length: 279 a.a.        Molecular weight: 30235.89 Da        Isoelectric Point: 7.1024

>NTDB_id=318 STER_RS00295 WP_011680595.1 45089..45928(-) (cbpD) [Streptococcus thermophilus LMD-9]
MKKKLLNYLILLACLATVSVSCSGSAQAAVLGDDYPFSWKYGGFGVDPWTMYWRQCTSFAAYRLSNTNGFTLPIGYGNAI
TWGNIARANGYRVDMNPAVGSIAWFSAGVNGAGHMGHVAWVAEVHGDQVTIEEYNYDAGQGPEKYHKRSFHKSQVSGYIH
FKDLESGTQNRNPTNSSIKVGDSVRFTGTFRVTSVSGNTITSQDLAGGTPTKHNIVDPSPVLEVDAQGNPTSDQYLNPGE
TFTIPGNFKVLAIDPPSDGILVQIGNLKTWVTQSVLEKI

Nucleotide


Download         Length: 840 bp        

>NTDB_id=318 STER_RS00295 WP_011680595.1 45089..45928(-) (cbpD) [Streptococcus thermophilus LMD-9]
ATGAAGAAAAAATTACTAAATTATCTCATACTGCTAGCTTGTCTAGCGACTGTTTCGGTTTCGTGCAGCGGCTCTGCTCA
GGCAGCTGTCCTCGGAGATGACTACCCTTTTTCTTGGAAATATGGTGGCTTCGGCGTGGACCCTTGGACTATGTACTGGC
GTCAGTGTACTTCCTTTGCGGCCTACCGCCTTAGCAACACCAATGGATTCACCTTACCTATAGGTTATGGAAATGCCATC
ACTTGGGGTAATATCGCACGTGCGAATGGCTACCGTGTTGACATGAATCCTGCCGTAGGGAGCATCGCCTGGTTCTCAGC
TGGTGTTAATGGAGCTGGTCACATGGGGCATGTCGCTTGGGTTGCAGAAGTACATGGTGATCAGGTAACTATTGAAGAAT
ACAACTACGATGCGGGTCAGGGGCCTGAGAAATATCACAAACGTAGCTTCCATAAGAGTCAAGTGTCTGGATATATCCAT
TTTAAAGACCTCGAATCAGGAACTCAAAATAGAAATCCTACTAATTCTTCTATAAAAGTTGGCGATTCAGTCCGATTCAC
AGGTACCTTCCGTGTCACCTCTGTGTCAGGAAATACCATCACCAGCCAAGATTTAGCTGGAGGTACACCAACCAAACACA
ATATCGTTGACCCTAGCCCCGTTCTTGAAGTTGATGCACAAGGTAATCCAACATCAGACCAGTACCTCAATCCTGGTGAG
ACCTTTACTATTCCAGGTAACTTCAAAGTTCTTGCCATTGACCCACCTAGCGATGGCATCCTCGTCCAAATCGGAAATCT
CAAGACTTGGGTAACCCAATCTGTCCTTGAAAAGATTTAA

Domains

Predicted by InterproScan.

CHAP domain (CHAP)

(48-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cbpD Streptococcus thermophilus LMG 18311

99.283

100

0.993

Multiple sequence alignment    



References

[1] Truls Johan Biørnstad et al. (2012) Deletion of the murein hydrolase CbpD reduces transformation efficiency in Streptococcus thermophilus. Microbiology (Reading, England) 158(Pt 4):877-885. [PMID: 22241050]