Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EYA88_RS18530 Genome accession   NZ_CP037760
Coordinates   539362..540870 (-) Length   502 a.a.
NCBI ID   WP_004195216.1    Uniprot ID   A0AAX1X7H0
Organism   Burkholderia pseudomallei strain BPs116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 534362..545870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYA88_RS18500 (EYA88_18500) - 535376..536008 (-) 633 WP_132056839.1 LON peptidase substrate-binding domain-containing protein -
  EYA88_RS18505 (EYA88_18505) rapZ 536035..536928 (-) 894 WP_004195227.1 RNase adapter RapZ -
  EYA88_RS18510 (EYA88_18510) hprK 537014..537982 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  EYA88_RS18515 (EYA88_18515) ptsN 538111..538620 (-) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EYA88_RS18520 (EYA88_18520) raiA 538853..539212 (-) 360 WP_004195219.1 ribosome-associated translation inhibitor RaiA -
  EYA88_RS18530 (EYA88_18530) rpoN/rpoN1 539362..540870 (-) 1509 WP_004195216.1 RNA polymerase factor sigma-54 Machinery gene
  EYA88_RS18535 (EYA88_18535) lptB 541039..541815 (-) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  EYA88_RS18540 (EYA88_18540) lptA 541812..542477 (-) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  EYA88_RS18545 (EYA88_18545) lptC 542496..543110 (-) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  EYA88_RS18550 (EYA88_18550) - 543116..543655 (-) 540 WP_004200146.1 HAD family hydrolase -
  EYA88_RS18555 (EYA88_18555) kdsD 543655..544638 (-) 984 WP_004530936.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54889.04 Da        Isoelectric Point: 4.8740

>NTDB_id=349941 EYA88_RS18530 WP_004195216.1 539362..540870(-) (rpoN/rpoN1) [Burkholderia pseudomallei strain BPs116]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=349941 EYA88_RS18530 WP_004195216.1 539362..540870(-) (rpoN/rpoN1) [Burkholderia pseudomallei strain BPs116]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAACATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment