Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EZV74_RS06670 Genome accession   NZ_CP037752
Coordinates   1205473..1207266 (+) Length   597 a.a.
NCBI ID   WP_014324326.1    Uniprot ID   -
Organism   Leuconostoc mesenteroides strain WiKim32     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1200473..1212266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZV74_RS06650 (EZV74_06630) - 1200648..1202090 (+) 1443 WP_014324323.1 ABC transporter substrate-binding protein/permease -
  EZV74_RS06655 (EZV74_06635) - 1202087..1202839 (+) 753 WP_014324324.1 amino acid ABC transporter ATP-binding protein -
  EZV74_RS06660 (EZV74_06640) - 1202906..1204351 (+) 1446 WP_011679289.1 ABC transporter substrate-binding protein/permease -
  EZV74_RS06665 (EZV74_06645) - 1204393..1205415 (+) 1023 WP_014324325.1 competence protein CoiA -
  EZV74_RS06670 (EZV74_06650) pepF 1205473..1207266 (+) 1794 WP_014324326.1 oligoendopeptidase F Regulator
  EZV74_RS06675 (EZV74_06655) - 1207371..1207766 (+) 396 WP_041775149.1 cytidine deaminase -
  EZV74_RS06680 (EZV74_06660) - 1207783..1208223 (+) 441 WP_014324328.1 nucleoside 2-deoxyribosyltransferase -
  EZV74_RS06685 (EZV74_06665) - 1208223..1209152 (+) 930 WP_014324329.1 phosphoglycerate dehydrogenase -
  EZV74_RS06690 (EZV74_06670) - 1209172..1209540 (+) 369 WP_014324330.1 helix-turn-helix domain-containing protein -
  EZV74_RS06695 (EZV74_06675) pheS 1209844..1210893 (+) 1050 WP_025267790.1 phenylalanine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 597 a.a.        Molecular weight: 67820.53 Da        Isoelectric Point: 4.6152

>NTDB_id=349910 EZV74_RS06670 WP_014324326.1 1205473..1207266(+) (pepF) [Leuconostoc mesenteroides strain WiKim32]
MSEKQLLRNQVPENLTWDLSSIFPTDEAWETAFNETRKSAQELSKYAGHVGDSADVLKEALEADLAVERQFEKVYVYAHQ
IYDQDTTNQKYSAYNARVQGLYAELSANTAFFQPEVLALSNETLDAYLATAGLKKYAHFFDVLKARKPHTLPAEQEALLA
GASDIFNASEQTFGALDNSDIVFGDVHTETGEIVPLTNGLYSLLLESKDRSLREEAFTTLYDSYIALQNTFASTLSSHVK
GHNFLAKTRHYDSARQAAVTANDIPESVFETLTKTVDANLPLLHRFVSLRKKILNVDDVHSYDLYVPLVDEINFEVTYEK
AKDIVLAALAPLGEDYIAIVQKAFDERWIDVVENKGKRTGAYSSGSYDTKPFILLNWQNNLNNVYTLAHEMGHSVHSYFT
RHNQEYHYGDYPIFLAEIASTTNEGLLTDYLLKTNDDPKFQAYVLNQYLDGFKGTVFRQTQFAEFEQWIHEQSAAGVALT
ADEMSTFYGSLNQKFYGSDLFPDEAIAYEWARIPHFYYNFYVYQYSTGEAAATTLAERILTQNGAEDYKNYLKAGNSDYA
LNVIKKAGVNMSQPDYLNEAFKVFEERLNEFEKIMLK

Nucleotide


Download         Length: 1794 bp        

>NTDB_id=349910 EZV74_RS06670 WP_014324326.1 1205473..1207266(+) (pepF) [Leuconostoc mesenteroides strain WiKim32]
ATGTCTGAAAAACAACTATTACGTAATCAAGTACCAGAAAATCTTACATGGGATCTATCCTCAATTTTTCCGACAGATGA
AGCTTGGGAAACCGCTTTTAATGAGACACGAAAGTCTGCACAAGAATTAAGCAAGTACGCTGGACACGTCGGGGATTCTG
CGGATGTTTTGAAAGAAGCCTTAGAAGCAGATTTAGCAGTAGAACGTCAATTCGAAAAAGTCTACGTATATGCTCATCAA
ATTTATGATCAAGATACAACTAATCAAAAGTATAGTGCGTATAATGCTCGTGTTCAAGGCTTATATGCTGAATTAAGTGC
GAATACAGCTTTCTTCCAGCCTGAAGTGTTAGCATTGTCAAACGAGACACTAGATGCATACTTAGCGACAGCTGGTTTGA
AAAAATATGCACACTTCTTTGACGTTTTAAAAGCTCGTAAACCGCATACGCTACCTGCAGAACAGGAAGCCTTACTAGCA
GGAGCAAGCGACATCTTTAATGCTTCTGAACAGACTTTTGGTGCATTGGATAATTCAGATATTGTTTTTGGTGATGTCCA
TACTGAAACAGGTGAAATTGTGCCATTGACAAATGGACTCTATTCATTGTTGTTGGAGTCTAAAGATCGTTCATTGCGTG
AGGAAGCGTTCACAACGCTTTATGATAGTTATATTGCTTTGCAAAATACATTTGCCTCAACGTTATCTTCACATGTCAAG
GGACATAACTTTTTGGCTAAAACTCGTCACTATGATTCAGCTCGTCAAGCTGCTGTAACAGCCAATGATATTCCTGAGTC
AGTTTTTGAAACATTAACCAAAACAGTTGATGCCAATCTGCCATTACTACACCGTTTTGTATCATTACGTAAAAAAATTC
TTAATGTTGACGATGTACACTCTTACGATTTATATGTACCACTTGTTGATGAAATTAATTTTGAAGTAACATATGAAAAG
GCAAAAGACATTGTTTTGGCGGCTTTGGCACCGTTAGGCGAGGATTACATCGCTATTGTTCAAAAGGCTTTTGATGAACG
ATGGATTGACGTGGTTGAAAACAAGGGGAAGCGTACTGGCGCTTATTCAAGCGGTTCGTATGATACAAAACCATTCATTT
TGCTTAACTGGCAGAACAATTTGAATAATGTTTATACGTTGGCTCATGAAATGGGACATTCGGTTCATTCATACTTTACA
CGCCATAATCAAGAGTATCATTACGGCGATTATCCAATATTCTTAGCTGAAATTGCTTCAACTACTAATGAGGGATTATT
AACGGATTATTTGCTAAAGACAAATGATGATCCCAAGTTTCAGGCCTACGTATTGAACCAGTATTTAGATGGATTCAAAG
GAACAGTTTTCCGCCAAACTCAATTTGCTGAGTTTGAGCAATGGATTCATGAACAAAGTGCGGCTGGAGTTGCTTTGACA
GCTGATGAGATGAGTACATTCTATGGATCGTTGAATCAAAAGTTTTATGGATCGGACTTATTTCCAGATGAAGCTATTGC
TTATGAATGGGCACGTATTCCACATTTTTATTACAATTTCTATGTTTATCAATATTCGACAGGCGAAGCTGCTGCCACAA
CTTTGGCAGAACGCATACTAACACAAAATGGTGCTGAGGATTATAAGAACTATCTGAAAGCAGGTAATTCAGATTATGCT
TTAAATGTGATCAAAAAAGCTGGCGTTAACATGAGTCAACCGGACTATTTAAATGAAGCTTTCAAAGTGTTTGAAGAGCG
ATTAAATGAGTTTGAAAAAATCATGTTAAAGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(115-183)

Peptidase family M3 (Peptidase_M3)

(204-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

52.445

99.33

0.521

Multiple sequence alignment