Detailed information NTG0349726

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: GJ664_RS08520
• Genome accession: NZ_CP046037
• Coordinates: 1701525..1705268 (+)
• Length: 1247 a.a.
• NCBI ID: WP_016265072.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain CBA3614
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1696525..1710268

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJ664_RS08510 (GJ664_08510)	mvk	1696757..1697704 (-)	948	WP_025015793.1	mevalonate kinase	-
GJ664_RS08515 (GJ664_08515)	-	1697981..1701541 (+)	3561	WP_016265071.1	PD-(D/E)XK nuclease family protein	-
GJ664_RS08520 (GJ664_08520)	addA	1701525..1705268 (+)	3744	WP_016265072.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
GJ664_RS08525 (GJ664_08525)	-	1705334..1708168 (+)	2835	WP_016265073.1	helicase C-terminal domain-containing protein	-
GJ664_RS08530 (GJ664_08530)	-	1708246..1708722 (+)	477	WP_011374615.1	DUF5590 domain-containing protein	-
GJ664_RS08535 (GJ664_08535)	asnS	1708833..1710131 (+)	1299	WP_011374616.1	asparagine--tRNA ligase	-

Sequence

Protein

• Length: 1247 a.a.
• Molecular weight: 141571.43 Da
• Isoelectric Point: 4.7686

>NTDB_id=349726 GJ664_RS08520 WP_016265072.1 1701525..1705268(+) (addA) [Latilactobacillus sakei strain CBA3614]
MAILSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILNGTDVDTLLVVTFTEAAATEMRQRIQVALRDAS
EKATEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKE
NEQDTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNNLPKSYAVDEAGLTASDYFQKKILPILQQTITECL
EALQEAEQLSQTSENLMIYAPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDD
VKKKIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQ
QKFSEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFR
SRRNIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRI
QAMMTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQD
IPLVSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDL
IWTIYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIH
GSKGLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRA
EQRLIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLIM
TNETLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQL
DLAAEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKINL
NHLLTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQ
EAAIERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3744 bp

>NTDB_id=349726 GJ664_RS08520 WP_016265072.1 1701525..1705268(+) (addA) [Latilactobacillus sakei strain CBA3614]
ATGGCAATCTTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATC
CGCATCAGCTGGTTCAGGGAAGACATCCGTTCTTGTTGCGCGGGTCATCCAAAAAATCCTAAACGGGACCGATGTCGATA
CCTTACTTGTTGTGACGTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGTAGCACTCCGCGACGCCAGT
GAGAAAGCAACAGAACCAGCCGTTAAGCAACGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATTAGTACACT
GCATGCCTTTTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATA
CCGCAGAACGACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAA
AATGAGCAAGATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTGACCGACATTGT
TTTTGAGTTGCTCGATTTTGCAAACGCCAATCCAAACCCGGTTAAATGGTTAAATAACCTTCCCAAAAGTTATGCCGTTG
ACGAAGCTGGTTTGACGGCCAGTGATTATTTCCAAAAAAAAATATTACCGATTTTACAGCAAACCATCACCGAATGTTTG
GAAGCCTTACAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATCTATGCCCCTCAAATTGCAACTACGCA
AGCAGCCTTAACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCACAGC
TGGGGCCTGCTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGATGAC
GTCAAAAAGAAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCAAGC
TGGCCAATTGGTTCAAAAATTGGTGAGCGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCCGAAAAAGAACGACGTCATT
TATTGGACTTTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGCAGAGGATTCGCCGGCAAGGGCCTTTTACCAA
CAAAAATTTAGCGAAGTGCTAGTTGATGAATATCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTC
TGATCATCCACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTAT
TTAAGAATAAGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGT
TCGCGCCGCAATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTACGA
CGAAAATGCGGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATG
AAACTAAGCCGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATT
CAAGCCATGATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGT
TTTACTAGCCCCAACCCGTGGTAATAATTTGTTTATCCTGGATTATTTCAAACGCTTCGGTTTACCGGTTGTGATTAAGG
ATGCGCAAAACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGATTGATAATCCTAACCAAGAT
ATTCCATTGGTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAA
GACGGATGATTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAACGAGCAGAAAGTCGGGCATTTAGGCCAACAGG
TCATGCAGAAGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATTGCTCGCCAACAACCGATTGTGGACTTA
ATTTGGACGATCTACCAAGAAACTGGCTTTTTAGACTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTT
ACACGCGCTTTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTGAAC
GTCTCCAGAAACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATTCAC
GGGAGTAAGGGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGTCGCTTTAACCAACAGGATTTACAACGCTC
ATATGTTTTGGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGCCAG
AGTTAGCGATTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCG
GAACAACGGTTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTAC
CCAACAAATATTAGATGATGGGGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATC
CACAAGCTAAAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATTATG
ACTAATGAAACCTTACTTGGTGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGCACT
TCAAACAATTGATTTAAACAGTGGGATAGCAACTGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCCA
CCCAAACGACGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGGTTGGGACAATTA
GACCTAGCGGCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGT
TCAACAACCAACGGCAGCAGAAGTAGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGC
TAGCGAGTTTGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTT
AATCACCTATTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCC
ATTTTCGTTGTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATGGGA
TTATGGATGGTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAA
GAAGCGGCGATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGCCCA
AAAGCCAGTGACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (10-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1067-1230)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-887)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.51	100	0.382