Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E0Z08_RS15820 Genome accession   NZ_CP037424
Coordinates   3198415..3199215 (-) Length   266 a.a.
NCBI ID   WP_131230604.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain M19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3193415..3204215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0Z08_RS15800 (E0Z08_15800) - 3194496..3195194 (-) 699 WP_005401704.1 YfgM family protein -
  E0Z08_RS15805 (E0Z08_15805) hisS 3195222..3196517 (-) 1296 WP_062031308.1 histidine--tRNA ligase -
  E0Z08_RS15810 (E0Z08_15810) ispG 3196514..3197629 (-) 1116 WP_130235007.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E0Z08_RS15815 (E0Z08_15815) - 3197648..3198424 (-) 777 WP_130235008.1 helix-turn-helix domain-containing protein -
  E0Z08_RS15820 (E0Z08_15820) pilF 3198415..3199215 (-) 801 WP_131230604.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E0Z08_RS15825 (E0Z08_15825) rlmN 3199232..3200467 (-) 1236 WP_130235009.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E0Z08_RS15830 (E0Z08_15830) ndk 3200590..3201021 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  E0Z08_RS15835 (E0Z08_15835) iscX 3201132..3201332 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  E0Z08_RS15840 (E0Z08_15840) - 3201423..3201974 (-) 552 WP_130235010.1 phosphatase PAP2 family protein -
  E0Z08_RS15845 (E0Z08_15845) - 3202167..3203447 (-) 1281 WP_130235011.1 glycosyltransferase family 4 protein -
  E0Z08_RS19045 - 3203529..3203999 (-) 471 WP_213075192.1 transposase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30108.11 Da        Isoelectric Point: 9.1263

>NTDB_id=349697 E0Z08_RS15820 WP_131230604.1 3198415..3199215(-) (pilF) [Acinetobacter johnsonii strain M19]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNMEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=349697 E0Z08_RS15820 WP_131230604.1 3198415..3199215(-) (pilF) [Acinetobacter johnsonii strain M19]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGAGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCACAAAATATGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCAGTTGGCGAGAAAAACTTAG
ATGCGCGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
TTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.657

100

0.692
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment