Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GJD96_RS19830 Genome accession   NZ_CP046009
Coordinates   3796470..3796910 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 1919D62     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3791470..3801910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJD96_RS19810 (GJD96_19825) aroP 3792468..3793841 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  GJD96_RS19815 (GJD96_19830) ampE 3793884..3794738 (-) 855 WP_096251386.1 beta-lactamase regulator AmpE -
  GJD96_RS19820 (GJD96_19835) ampD 3794735..3795286 (-) 552 WP_095885630.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GJD96_RS19825 (GJD96_19840) nadC 3795374..3796267 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GJD96_RS19830 (GJD96_19845) pilA 3796470..3796910 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  GJD96_RS19835 (GJD96_19850) pilB 3796920..3798305 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  GJD96_RS19840 (GJD96_19855) hofC 3798295..3799497 (+) 1203 WP_000157266.1 protein transport protein HofC -
  GJD96_RS19845 (GJD96_19860) guaC 3799532..3800575 (-) 1044 WP_001217338.1 GMP reductase -
  GJD96_RS24010 - 3800731..3800775 (-) 45 WP_120795372.1 protein YacM -
  GJD96_RS19850 (GJD96_19865) coaE 3800800..3801420 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=349558 GJD96_RS19830 WP_000360904.1 3796470..3796910(+) (pilA) [Escherichia coli strain 1919D62]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=349558 GJD96_RS19830 WP_000360904.1 3796470..3796910(+) (pilA) [Escherichia coli strain 1919D62]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment