Detailed information NTG0349315

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: EYB46_RS12125
• Genome accession: NZ_CP036518
• Coordinates: 2478567..2479712 (-)
• Length: 381 a.a.
• NCBI ID: WP_074534220.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain ANSB01E
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 2473567..2484712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EYB46_RS12100 (EYB46_12095)	-	2473850..2474283 (+)	434	Protein_2319	hypothetical protein	-
EYB46_RS12105 (EYB46_12100)	-	2474308..2476380 (-)	2073	WP_032875881.1	transglycosylase domain-containing protein	-
EYB46_RS12110 (EYB46_12105)	speE	2476580..2477410 (+)	831	WP_003151052.1	spermidine synthase	-
EYB46_RS12115 (EYB46_12110)	speB	2477469..2478341 (+)	873	WP_032875884.1	agmatinase	-
EYB46_RS12120 (EYB46_12115)	-	2478464..2478583 (-)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
EYB46_RS12125 (EYB46_12120)	rapF	2478567..2479712 (-)	1146	WP_074534220.1	Rap family tetratricopeptide repeat protein	Regulator
EYB46_RS12135 (EYB46_12130)	-	2479943..2481298 (+)	1356	WP_032875887.1	YncE family protein	-
EYB46_RS12140 (EYB46_12135)	-	2481338..2482714 (+)	1377	WP_032875890.1	YncE family protein	-
EYB46_RS12145 (EYB46_12140)	-	2482721..2483233 (-)	513	Protein_2327	bacteriocin biosynthesis protein AlbG	-
EYB46_RS12150 (EYB46_12145)	-	2483387..2483815 (+)	429	WP_032869448.1	DUF1934 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45737.21 Da
• Isoelectric Point: 5.0614

>NTDB_id=349315 EYB46_RS12125 WP_074534220.1 2478567..2479712(-) (rapF) [Bacillus velezensis strain ANSB01E]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDAEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLKNIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLENKLMYADIEELALDVAKYYYTQEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=349315 EYB46_RS12125 WP_074534220.1 2478567..2479712(-) (rapF) [Bacillus velezensis strain ANSB01E]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGCAGAAAAATATAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGGACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGTCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAAAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTAGAATACTTGTATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAATAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACACAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAGGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (188-254)

• Tetratricopeptide repeat (TPR_8) (265-293)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	54.211	99.738	0.541