Detailed information NTG0349221

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: GI529_RS13030
• Genome accession: NZ_CP045977
• Coordinates: 2675693..2676685 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain INSC1002
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2670693..2681685

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GI529_RS13010 (GI529_13000)	otnK	2671468..2672730 (+)	1263	WP_000590371.1	3-oxo-tetronate kinase	-
GI529_RS13015 (GI529_13005)	ygbL	2672727..2673365 (+)	639	WP_001278994.1	aldolase	-
GI529_RS13020 (GI529_13010)	ygbM	2673370..2674146 (+)	777	WP_001136925.1	HPr family phosphocarrier protein	-
GI529_RS13025 (GI529_13015)	ygbN	2674235..2675599 (+)	1365	WP_000104459.1	GntP family transporter	-
GI529_RS13030 (GI529_13020)	rpoS	2675693..2676685 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
GI529_RS13035 (GI529_13025)	nlpD	2676748..2677842 (-)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
GI529_RS13040 (b2743)	pcm	2678027..2678653 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
GI529_RS13045 (b2744)	surE	2678647..2679408 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
GI529_RS13050 (GI529_13040)	truD	2679389..2680438 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
GI529_RS13055 (b2746)	ispF	2680435..2680914 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
GI529_RS13060 (b2747)	ispD	2680914..2681624 (-)	711	WP_000246144.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=349221 GI529_RS13030 WP_000081550.1 2675693..2676685(-) (rpoS) [Escherichia coli strain INSC1002]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=349221 GI529_RS13030 WP_000081550.1 2675693..2676685(-) (rpoS) [Escherichia coli strain INSC1002]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715