Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GIY05_RS01920 Genome accession   NZ_CP045916
Coordinates   423021..424355 (+) Length   444 a.a.
NCBI ID   WP_003158456.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CF39S     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418021..429355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIY05_RS01900 - 418858..419424 (-) 567 WP_003084491.1 hypothetical protein -
  GIY05_RS01905 rsmD 419427..420023 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  GIY05_RS01910 - 420023..421510 (-) 1488 WP_003102862.1 pitrilysin family protein -
  GIY05_RS01915 - 421503..422900 (-) 1398 WP_003084498.1 pitrilysin family protein -
  GIY05_RS01920 pilA 423021..424355 (+) 1335 WP_003158456.1 signal recognition particle-docking protein FtsY Machinery gene
  GIY05_RS01925 ftsE 424352..425023 (+) 672 WP_003100975.1 cell division ATP-binding protein FtsE -
  GIY05_RS01930 ftsX 425023..426030 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  GIY05_RS01935 rpoH 426144..426998 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  GIY05_RS01940 - 427063..427668 (+) 606 WP_003084511.1 hypothetical protein -
  GIY05_RS01945 mtgA 427673..428404 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GIY05_RS01950 - 428441..428818 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  GIY05_RS01955 thiS 428921..429121 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 47189.58 Da        Isoelectric Point: 4.6728

>NTDB_id=348428 GIY05_RS01920 WP_003158456.1 423021..424355(+) (pilA) [Pseudomonas aeruginosa strain CF39S]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPPAPVAE
APLAGDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIGEGMASL
FLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDVAREPYV
ILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQAAKARG
IDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLDGTAKGG
VIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=348428 GIY05_RS01920 WP_003158456.1 423021..424355(+) (pilA) [Pseudomonas aeruginosa strain CF39S]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGCCGGCGCCGGTTGCCGAA
GCGCCGCTCGCCGGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGCCCCGAGGCGCCCGTCGCACAACCCGAGCC
TATCCTCGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCGCTCCGGCAGTCAGCGAGCCGGCGA
CCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGCATCGGCGAAGGCATGGCCAGCCTG
TTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGACCGCCGACGTCGGCGTCGA
GGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACAGCGGCGCGCTGTACAAGG
CGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTCGCGCGCGAGCCCTATGTG
ATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAAGCTGCAACTCGAGGGCAA
GAAGGTCATGCTGGCTGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCTGGGGCGAGCGCAACCGCA
TCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAGGCCGCCAAGGCCCGCGGC
ATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGAGCTGAAGAAGGTGCGCCG
GGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCACCGGGCAGAACGCCATCA
ACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGACGGCACCGCCAAGGGCGGG
GTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGGGATCGACGATCTCCGTAC
CTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(154-219)

SRP54-type protein, GTPase domain (SRP54)

(239-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.897

96.396

0.462

Multiple sequence alignment