Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   GIX96_RS21020 Genome accession   NZ_CP045863
Coordinates   4207827..4208495 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain ATCC 43890     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4202827..4213495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIX96_RS20990 (GIX96_20990) rfaQ 4203005..4204063 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  GIX96_RS20995 (GIX96_20995) waaA 4204476..4205753 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  GIX96_RS21000 (GIX96_21000) coaD 4205761..4206240 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  GIX96_RS21005 (GIX96_21005) mutM 4206279..4207088 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  GIX96_RS21010 (GIX96_21010) rpmG 4207186..4207353 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  GIX96_RS21015 (GIX96_21015) rpmB 4207374..4207610 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  GIX96_RS21020 (GIX96_21020) radC 4207827..4208495 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  GIX96_RS21025 (GIX96_21025) coaBC 4208667..4209887 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  GIX96_RS21030 (GIX96_21030) dut 4209865..4210323 (+) 459 WP_000976070.1 dUTP diphosphatase -
  GIX96_RS21035 (GIX96_21035) slmA 4210430..4211026 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  GIX96_RS21040 (GIX96_21040) pyrE 4211063..4211704 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  GIX96_RS21045 (GIX96_21045) rph 4211770..4212486 (-) 717 WP_001247093.1 ribonuclease PH -
  GIX96_RS21050 (GIX96_21050) yicC 4212613..4213476 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=348311 GIX96_RS21020 WP_001297375.1 4207827..4208495(-) (radC) [Escherichia coli O157:H7 strain ATCC 43890]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=348311 GIX96_RS21020 WP_001297375.1 4207827..4208495(-) (radC) [Escherichia coli O157:H7 strain ATCC 43890]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment