Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GIJ02_RS01355 Genome accession   NZ_CP045827
Coordinates   298237..299733 (+) Length   498 a.a.
NCBI ID   WP_001040651.1    Uniprot ID   -
Organism   Escherichia coli strain AUSMDU00014361     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 293237..304733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIJ02_RS01325 (GIJ02_01325) tusA 293484..293729 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  GIJ02_RS01330 (GIJ02_01330) zntA 293831..296029 (-) 2199 WP_000106580.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  GIJ02_RS01335 (GIJ02_01335) yhhN 296103..296729 (-) 627 WP_000964718.1 lysoplasmalogenase -
  GIJ02_RS01340 (GIJ02_01340) yhhM 296870..297229 (+) 360 WP_000042873.1 DUF2500 domain-containing protein -
  GIJ02_RS01345 (GIJ02_01345) yhhL 297232..297501 (-) 270 WP_001295207.1 DUF1145 family protein -
  GIJ02_RS01350 (GIJ02_01350) rsmD 297491..298087 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  GIJ02_RS01355 (GIJ02_01355) pilA 298237..299733 (+) 1497 WP_001040651.1 signal recognition particle-docking protein FtsY Machinery gene
  GIJ02_RS01360 (GIJ02_01360) ftsE 299736..300404 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  GIJ02_RS01365 (GIJ02_01365) ftsX 300397..301455 (+) 1059 WP_001042001.1 permease-like cell division protein FtsX -
  GIJ02_RS01370 (GIJ02_01370) rpoH 301700..302554 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  GIJ02_RS01375 (GIJ02_01375) livJ 302825..303928 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  GIJ02_RS01380 (GIJ02_01380) panM 304127..304510 (-) 384 WP_000778789.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54671.46 Da        Isoelectric Point: 4.1468

>NTDB_id=348111 GIJ02_RS01355 WP_001040651.1 298237..299733(+) (pilA) [Escherichia coli strain AUSMDU00014361]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVAEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=348111 GIJ02_RS01355 WP_001040651.1 298237..299733(+) (pilA) [Escherichia coli strain AUSMDU00014361]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCAGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCAGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTAGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATTGCCCAGGA
GCAAGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGTGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACTACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGTACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment