Detailed information NTG0348062

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EYB55_RS15325
• Genome accession: NZ_CP036335
• Coordinates: 3066240..3067040 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain BP327
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3061240..3072040

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EYB55_RS15290 (EYB55_15290)	gspH	3061396..3061911 (+)	516	WP_131079634.1	type II secretion system minor pseudopilin GspH	-
EYB55_RS15295 (EYB55_15295)	gspI	3061908..3062273 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
EYB55_RS15300 (EYB55_15300)	gspJ	3062270..3062866 (+)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
EYB55_RS15305 (EYB55_15305)	gspK	3062863..3063849 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
EYB55_RS15310 (EYB55_15310)	gspL	3063836..3065029 (+)	1194	WP_004194919.1	type II secretion system protein GspL	-
EYB55_RS15315 (EYB55_15315)	-	3065022..3065507 (+)	486	WP_004145896.1	type II secretion system protein M	-
EYB55_RS15320 (EYB55_15320)	-	3065497..3066243 (+)	747	WP_004178605.1	type II secretion system protein N	-
EYB55_RS15325 (EYB55_15325)	pilD	3066240..3067040 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
EYB55_RS15330 (EYB55_15330)	hrpB	3067088..3069517 (-)	2430	WP_131079635.1	ATP-dependent helicase HrpB	-
EYB55_RS15335 (EYB55_15335)	thpR	3069590..3070126 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
EYB55_RS15340 (EYB55_15340)	sfsA	3070126..3070842 (+)	717	WP_020324420.1	DNA/RNA nuclease SfsA	-
EYB55_RS15345 (EYB55_15345)	dksA	3071005..3071460 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=348062 EYB55_RS15325 WP_004145898.1 3066240..3067040(+) (pilD) [Klebsiella pneumoniae strain BP327]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=348062 EYB55_RS15325 WP_004145898.1 3066240..3067040(+) (pilD) [Klebsiella pneumoniae strain BP327]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCATGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425