Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MSIP34_RS06595 Genome accession   NC_012969
Coordinates   1403296..1404384 (-) Length   362 a.a.
NCBI ID   WP_013442155.1    Uniprot ID   C6XDB5
Organism   Methylovorus glucosotrophus SIP3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1398296..1409384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS06570 (Msip34_1289) thiD 1398373..1399218 (+) 846 WP_015830014.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  MSIP34_RS06575 (Msip34_1290) miaA 1399307..1400263 (+) 957 WP_015830015.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  MSIP34_RS06580 (Msip34_1291) dapC 1400300..1401481 (-) 1182 WP_015830016.1 succinyldiaminopimelate transaminase -
  MSIP34_RS06585 (Msip34_1292) - 1401564..1402442 (+) 879 WP_015830017.1 DMT family transporter -
  MSIP34_RS06590 (Msip34_1293) - 1402399..1403196 (-) 798 WP_015830018.1 intradiol ring-cleavage dioxygenase -
  MSIP34_RS06595 (Msip34_1294) pilU 1403296..1404384 (-) 1089 WP_013442155.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MSIP34_RS06600 (Msip34_1295) - 1404608..1406965 (+) 2358 WP_015830019.1 EAL domain-containing protein -
  MSIP34_RS06605 (Msip34_1296) - 1407043..1409136 (-) 2094 WP_015830020.1 glycosyltransferase family 41 protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40226.45 Da        Isoelectric Point: 4.9637

>NTDB_id=34794 MSIP34_RS06595 WP_013442155.1 1403296..1404384(-) (pilU) [Methylovorus glucosotrophus SIP3-4]
MAAIDITPVLRFMVDKNGSDLFFSTGTPIHIEIEGKTVPVNTQVMAPGMVKEVAYALMSPEQIKEFEATLEMNFAYSVDE
IEDARFRVNIFRQRGDVAIVIRCIKALIPTFEALGLPPILKELIMKPRGLVLVVGSTGSGKSTTLASMIDYRNENTTGHI
LTLEDPIEFIHKHKKSIVDQREIGIDTLSFDNGLKNAMRQAPDVILIGEIRDMETMKHAMAYSETGHLCLATLHANNANQ
AIERIISFFPPEGKAGLLLGLSMNLVAIVSQRLIPGKLTKRAAAIEVLINTPYISELIQKSKLEEIKDVMADNNDIGMCT
FDQSLFRLYSNDRITEENALANADSRNDLSLRIRFADESEES

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=34794 MSIP34_RS06595 WP_013442155.1 1403296..1404384(-) (pilU) [Methylovorus glucosotrophus SIP3-4]
ATGGCAGCCATTGATATCACGCCAGTTTTGCGTTTCATGGTCGATAAAAACGGATCAGATCTTTTCTTCAGCACGGGCAC
CCCTATCCATATCGAGATTGAGGGCAAAACCGTGCCGGTGAACACGCAAGTGATGGCGCCGGGCATGGTAAAAGAGGTGG
CCTATGCCCTGATGTCCCCCGAGCAGATCAAGGAATTTGAAGCCACGCTGGAAATGAACTTTGCCTATAGCGTGGATGAA
ATCGAAGATGCGCGTTTCCGCGTCAATATCTTTCGCCAGCGTGGCGATGTCGCCATCGTCATTCGCTGTATCAAGGCGCT
GATTCCCACATTTGAAGCGCTCGGGCTGCCGCCTATCCTGAAAGAGCTGATCATGAAGCCACGTGGCCTGGTGCTGGTCG
TGGGTTCTACCGGGTCTGGCAAATCCACCACGCTCGCTTCCATGATCGACTACCGCAATGAAAATACCACCGGCCATATC
CTGACGCTGGAAGACCCGATCGAATTCATCCACAAGCACAAAAAATCCATCGTCGATCAGCGCGAAATCGGCATTGATAC
CCTGTCTTTTGACAATGGCCTGAAAAACGCCATGCGGCAGGCGCCGGATGTGATTTTGATAGGTGAAATTCGCGACATGG
AAACCATGAAGCATGCCATGGCCTACTCCGAAACCGGGCACTTGTGCCTGGCTACCTTGCATGCCAACAACGCCAACCAG
GCCATTGAGCGCATCATTTCCTTCTTCCCGCCCGAGGGCAAAGCCGGTTTGCTGCTGGGGCTTTCCATGAATCTGGTGGC
GATTGTTTCGCAGCGCCTGATCCCGGGCAAGCTGACCAAACGCGCAGCGGCTATCGAGGTGTTGATCAACACGCCTTATA
TCTCCGAGCTGATCCAGAAATCCAAGCTGGAAGAGATCAAGGATGTCATGGCGGACAACAACGATATCGGCATGTGCACT
TTCGACCAGTCGCTATTCCGCTTGTACAGCAACGACAGGATCACCGAGGAAAATGCGCTGGCAAATGCCGATTCCCGCAA
TGACCTGTCATTGCGCATCCGGTTTGCGGATGAGTCCGAGGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6XDB5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.352

98.895

0.528
  pilU Vibrio cholerae strain A1552

48.864

97.238

0.475
  pilU Acinetobacter baylyi ADP1

47.899

98.619

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.149

94.751

0.409
  pilT Legionella pneumophila strain Lp02

43.452

92.818

0.403
  pilT Legionella pneumophila strain ERS1305867

43.452

92.818

0.403
  pilT Acinetobacter baylyi ADP1

42.398

94.475

0.401
  pilT Pseudomonas aeruginosa PAK

42.059

93.923

0.395
  pilT Acinetobacter nosocomialis M2

42.059

93.923

0.395
  pilT Acinetobacter baumannii strain A118

41.765

93.923

0.392
  pilT Acinetobacter baumannii D1279779

41.765

93.923

0.392
  pilT Pseudomonas stutzeri DSM 10701

41.176

93.923

0.387
  pilT Neisseria meningitidis 8013

39.828

96.409

0.384
  pilT Neisseria gonorrhoeae MS11

39.828

96.409

0.384

Multiple sequence alignment