Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII77_RS13520 Genome accession   NZ_CP045825
Coordinates   2607000..2607410 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain 75     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2602000..2612410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII77_RS13490 (GII77_13490) - 2602087..2602227 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII77_RS13495 (GII77_13495) yqeF 2602533..2603264 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  GII77_RS13500 (GII77_13500) cwlH 2603516..2604268 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII77_RS13505 (GII77_13505) yqeD 2604455..2605081 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  GII77_RS13510 (GII77_13510) gnd 2605100..2605993 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII77_RS13515 (GII77_13515) yqeB 2606245..2606967 (+) 723 WP_010886572.1 hypothetical protein -
  GII77_RS13520 (GII77_13520) nucA/comI 2607000..2607410 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII77_RS13525 (GII77_13525) - 2607606..2607956 (+) 351 Protein_2637 sigma-70 family RNA polymerase sigma factor -
  GII77_RS13530 (GII77_13530) - 2608096..2608566 (+) 471 WP_015251668.1 MarR family transcriptional regulator -
  GII77_RS13535 (GII77_13535) - 2608716..2610128 (+) 1413 WP_015251667.1 MDR family MFS transporter -
  GII77_RS13540 (GII77_13540) spoIVCA 2610315..2611775 (-) 1461 WP_235701260.1 site-specific DNA recombinase SpoIVCA -
  GII77_RS21695 - 2611733..2611911 (-) 179 Protein_2641 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=347899 GII77_RS13520 WP_009967785.1 2607000..2607410(-) (nucA/comI) [Bacillus subtilis strain 75]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=347899 GII77_RS13520 WP_009967785.1 2607000..2607410(-) (nucA/comI) [Bacillus subtilis strain 75]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529