Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MSIP34_RS01065 Genome accession   NC_012969
Coordinates   229102..230556 (-) Length   484 a.a.
NCBI ID   WP_015829204.1    Uniprot ID   C6X8I1
Organism   Methylovorus glucosotrophus SIP3-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224102..235556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS01035 (Msip34_0196) ptsP 224361..226115 (-) 1755 WP_013441022.1 phosphoenolpyruvate--protein phosphotransferase -
  MSIP34_RS01040 (Msip34_0197) - 226123..226392 (-) 270 WP_013441023.1 HPr family phosphocarrier protein -
  MSIP34_RS01045 (Msip34_0198) - 226385..226792 (-) 408 WP_013441024.1 PTS sugar transporter subunit IIA -
  MSIP34_RS01050 (Msip34_0199) rapZ 226843..227679 (-) 837 WP_041362502.1 RNase adapter RapZ -
  MSIP34_RS01055 (Msip34_0200) hprK 227697..228662 (-) 966 WP_013441026.1 HPr(Ser) kinase/phosphatase -
  MSIP34_RS01060 (Msip34_0201) hpf 228745..229074 (-) 330 WP_013441027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MSIP34_RS01065 (Msip34_0202) rpoN/rpoN1 229102..230556 (-) 1455 WP_015829204.1 RNA polymerase factor sigma-54 Machinery gene
  MSIP34_RS01070 (Msip34_0203) lptB 230581..231303 (-) 723 WP_013441029.1 LPS export ABC transporter ATP-binding protein -
  MSIP34_RS01075 (Msip34_0204) lptA 231360..231917 (-) 558 WP_013441030.1 lipopolysaccharide transport periplasmic protein LptA -
  MSIP34_RS01080 (Msip34_0205) lptC 231898..232653 (-) 756 WP_015829205.1 LPS export ABC transporter periplasmic protein LptC -
  MSIP34_RS01085 (Msip34_0206) - 232663..233187 (-) 525 WP_013441032.1 HAD family hydrolase -
  MSIP34_RS01090 (Msip34_0207) - 233189..234187 (-) 999 WP_015829206.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 54592.25 Da        Isoelectric Point: 5.0208

>NTDB_id=34785 MSIP34_RS01065 WP_015829204.1 229102..230556(-) (rpoN/rpoN1) [Methylovorus glucosotrophus SIP3-4]
MKQSLQLKFSQHLALTPQLQQSIRLLQLSTQELNQELETLLQANPLLERADKIEEPLEIPDNFIAAPVAPEPSVAATPEE
APRQDEFGGDDYFEFGSSSGSNRWEDSSSGNDDDNDFSYQEAVSLSLRDHLLGQLKLLPLSERDQTLSMLLVDTITDDGY
LEQSLEELADMLPPELEIDMLELQTALKHIQNLDPPGIGARNLAECLSLQLRALPPDTPYVDLAIKVAETHLPLLAARDY
AKLKKLLACDDDVLREVQQLVLQLNPRPGGEFSKITSDHYIQHEIVVKKIKGVWLAHLNDEVVPKLRINQLYAGILKRNR
ESSSQYLMSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGEVAMRPLVLREIADELGLHESTVSRVTTRKYMLT
PRGVYELKYFFGSHVATESGGACSATAIRALIKQMVSEENPKKPLSDNQITDILGKQGIVVARRTIAKYRESLQIPAANQ
RRSL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=34785 MSIP34_RS01065 WP_015829204.1 229102..230556(-) (rpoN/rpoN1) [Methylovorus glucosotrophus SIP3-4]
ATGAAGCAAAGCTTACAACTCAAATTTTCGCAACACCTGGCGCTGACTCCGCAACTGCAGCAGTCCATCCGTTTGTTGCA
GCTATCCACGCAGGAGCTCAATCAGGAGCTGGAAACCCTGCTGCAGGCGAATCCATTGCTGGAACGCGCTGACAAGATTG
AAGAACCGCTGGAAATCCCCGACAACTTCATTGCGGCGCCAGTAGCCCCTGAGCCCAGCGTGGCCGCTACACCGGAGGAA
GCTCCCCGCCAGGATGAATTTGGTGGCGATGACTATTTTGAATTCGGCAGCAGCAGCGGCAGCAACCGCTGGGAAGACAG
CAGCTCCGGCAATGACGATGACAATGATTTCAGCTATCAGGAAGCGGTCAGCCTCAGCCTGCGCGATCATCTGCTAGGTC
AGCTCAAGCTGTTGCCACTGTCCGAGCGCGACCAGACCTTGTCCATGCTGCTGGTCGATACCATTACCGACGACGGTTAT
CTGGAGCAGTCGCTGGAAGAGCTGGCCGACATGCTGCCACCGGAGCTCGAAATCGACATGCTGGAGTTGCAGACGGCGCT
CAAGCATATCCAGAACCTGGACCCGCCCGGCATAGGTGCCCGCAATCTGGCAGAGTGCCTGTCCTTGCAATTGCGCGCCT
TGCCACCAGATACGCCTTATGTGGATCTCGCTATCAAGGTTGCCGAAACGCACTTGCCCCTGCTGGCAGCCCGCGATTAC
GCAAAACTGAAAAAATTGCTGGCCTGCGACGATGATGTATTGCGCGAAGTGCAACAGCTGGTGCTGCAACTCAATCCCCG
CCCAGGTGGTGAATTCAGCAAAATCACGTCTGATCATTACATTCAGCACGAAATCGTGGTGAAGAAAATCAAAGGTGTCT
GGCTGGCCCATCTGAACGACGAAGTCGTGCCCAAGCTGCGCATCAACCAGCTGTATGCCGGCATCCTCAAGCGCAACCGC
GAAAGCTCGAGCCAATACCTGATGAGCCAGATGCAGGAAGCCAAGTGGATGATCAAGAACATCCAGCAGCGTTTCTCCAC
CATCCTGCGCGTTTCTCAGGCGATTGTGGATCGCCAGCGCAATTTCTTTGAGCACGGCGAGGTCGCCATGCGCCCCCTGG
TGCTGCGCGAAATTGCCGATGAGCTGGGCCTGCACGAATCCACGGTGTCACGCGTTACCACGCGCAAGTACATGCTCACC
CCACGCGGAGTTTACGAGCTTAAATATTTCTTTGGCAGCCATGTGGCTACCGAATCCGGGGGCGCTTGTTCCGCCACTGC
CATACGCGCCCTGATCAAACAAATGGTCAGCGAAGAGAACCCGAAAAAGCCGCTGTCGGATAACCAGATCACGGATATCC
TGGGTAAACAGGGCATCGTGGTTGCTCGTCGCACCATTGCCAAATACCGCGAGTCTTTGCAGATACCCGCGGCCAACCAG
CGCAGATCGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(123-312)

Sigma-54, DNA binding domain (Sigma54_DBD)

(325-482)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X8I1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.36

100

0.558

Multiple sequence alignment