Detailed information NTG0347811

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GII77_RS01680
• Genome accession: NZ_CP045825
• Coordinates: 305338..306459 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain 75
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 300338..311459

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII77_RS01655 (GII77_01655)	ycdA	300343..301407 (-)	1065	WP_015252891.1	DUF4352 domain-containing protein	-
GII77_RS01660 (GII77_01660)	-	301430..301561 (-)	132	WP_003246334.1	hypothetical protein	-
GII77_RS01665 (GII77_01665)	ycdB	301735..303156 (+)	1422	WP_015252890.1	YcdB/YcdC domain-containing protein	-
GII77_RS01670 (GII77_01670)	ycdC	303343..304704 (+)	1362	WP_015252889.1	YcdB/YcdC domain-containing protein	-
GII77_RS01675 (GII77_01675)	cwlK	304712..305215 (-)	504	WP_015252888.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
GII77_RS01680 (GII77_01680)	rapF	305338..306459 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
GII77_RS01685 (GII77_01685)	ycdF	306566..307342 (+)	777	WP_003234739.1	SDR family oxidoreductase	-
GII77_RS01690 (GII77_01690)	ycdG	307367..309052 (+)	1686	WP_015252887.1	alpha-glucosidase	-
GII77_RS01695 (GII77_01695)	znuA	309240..310199 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
GII77_RS01700 (GII77_01700)	znuC	310254..310949 (+)	696	WP_014475714.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=347811 GII77_RS01680 WP_003246478.1 305338..306459(+) (rapF) [Bacillus subtilis strain 75]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=347811 GII77_RS01680 WP_003246478.1 305338..306459(+) (rapF) [Bacillus subtilis strain 75]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATACGATTTAGAAGATATGGAAGAGAACCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGACACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCCGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCGTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGCT
GGAACATTACGAGTCAGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405