Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MMOL_RS10945 Genome accession   NC_012968
Coordinates   2340740..2342317 (-) Length   525 a.a.
NCBI ID   WP_015833101.1    Uniprot ID   C6WT04
Organism   Methylotenera mobilis JLW8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2335740..2347317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMOL_RS10915 (Mmol_2158) ptsP 2335888..2337654 (-) 1767 WP_015833095.1 phosphoenolpyruvate--protein phosphotransferase -
  MMOL_RS10920 (Mmol_2159) - 2337651..2337920 (-) 270 WP_015833096.1 HPr family phosphocarrier protein -
  MMOL_RS10925 (Mmol_2160) - 2337950..2338372 (-) 423 WP_015833097.1 PTS sugar transporter subunit IIA -
  MMOL_RS10930 (Mmol_2161) rapZ 2338392..2339225 (-) 834 WP_015833098.1 RNase adapter RapZ -
  MMOL_RS10935 (Mmol_2162) hprK 2339288..2340229 (-) 942 WP_015833099.1 HPr(Ser) kinase/phosphatase -
  MMOL_RS10940 (Mmol_2163) hpf 2340268..2340594 (-) 327 WP_015833100.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MMOL_RS10945 (Mmol_2164) rpoN/rpoN1 2340740..2342317 (-) 1578 WP_015833101.1 RNA polymerase factor sigma-54 Machinery gene
  MMOL_RS10950 (Mmol_2165) lptB 2342314..2343036 (-) 723 WP_015833102.1 LPS export ABC transporter ATP-binding protein -
  MMOL_RS10955 (Mmol_2166) lptA 2343086..2343748 (-) 663 WP_148207869.1 lipopolysaccharide transport periplasmic protein LptA -
  MMOL_RS10960 (Mmol_2167) lptC 2343759..2344478 (-) 720 WP_015833104.1 LPS export ABC transporter periplasmic protein LptC -
  MMOL_RS10965 (Mmol_2168) - 2344547..2345053 (-) 507 WP_049764529.1 KdsC family phosphatase -
  MMOL_RS10970 (Mmol_2169) - 2345108..2346133 (-) 1026 WP_015833106.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 58731.16 Da        Isoelectric Point: 4.4967

>NTDB_id=34780 MMOL_RS10945 WP_015833101.1 2340740..2342317(-) (rpoN/rpoN1) [Methylotenera mobilis JLW8]
MKQGLQLKFSQNLSLTPQLQQAIRLLQLSTLELNQEIDVLMQTNPLLERGDDTEDEYGNAVEPVADGADLSATSPESAEA
KPEFENFDAEYAESASEFNGAESANASVADVSDTADFDVPSTDYADTFSAEFNDEFDEFSNGSRWDENTMPADDDSDFKP
QETLQTSLREHLLSQLKLMPLSERDQSLALMLVDSINDDGYLEQSLEEIAELMPEELEIDAIELQTALRHVQHLDPPGVG
ARSLSECLLLQLAVLPKSTPHLAMADLVAKHHLQVLASRDFVKLRKELGCDEATLKQVQQLITSLNPRPGSAFSHIGSEH
YIQHEVIVKKVKGIWIASLNDSVIPKLKINQLYAGILKRNRDSSSQYLQSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQ
RNFLEYGEVAMRPLVLREIADELDLHESTISRVTTNKYMLTPRGIFELKYFFGSSVATDAGGTCSATAIRALIKQLVDQE
NPRKPYSDNQITDLLAKQGIVVARRTIAKYRESMNIAPASLRKSL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=34780 MMOL_RS10945 WP_015833101.1 2340740..2342317(-) (rpoN/rpoN1) [Methylotenera mobilis JLW8]
ATGAAGCAAGGGTTACAACTAAAGTTTTCACAGAATCTATCGCTGACGCCGCAGTTGCAGCAGGCGATTCGCCTGTTGCA
ACTTTCAACGCTGGAGCTGAATCAAGAAATTGATGTGCTGATGCAAACTAATCCCCTGCTGGAACGGGGTGACGACACCG
AGGATGAGTACGGCAACGCCGTAGAGCCGGTGGCTGACGGAGCCGACTTATCTGCTACCAGCCCTGAGTCTGCAGAGGCT
AAGCCTGAGTTTGAAAATTTTGATGCTGAGTATGCGGAATCTGCGAGTGAGTTCAATGGCGCAGAGTCTGCAAATGCAAG
TGTTGCGGATGTGTCCGATACGGCTGATTTTGATGTGCCAAGCACAGATTACGCAGACACGTTTTCGGCTGAGTTTAATG
ATGAGTTTGATGAGTTTTCCAACGGCAGCCGTTGGGATGAAAACACCATGCCGGCGGATGATGACAGTGACTTCAAGCCG
CAGGAAACTTTGCAGACCAGTTTGCGTGAGCATTTGCTGTCCCAGTTAAAGTTGATGCCCTTGTCCGAGCGCGATCAATC
GCTTGCGTTGATGCTAGTAGATAGCATTAATGATGATGGTTACTTGGAGCAAAGCCTGGAGGAAATTGCCGAGCTGATGC
CAGAAGAGCTGGAGATAGATGCGATAGAGTTGCAAACAGCGTTGCGTCATGTGCAGCATTTGGATCCACCAGGCGTAGGT
GCGCGCAGCTTAAGTGAGTGCTTGCTACTGCAGCTTGCGGTGTTGCCGAAATCTACACCGCATTTGGCGATGGCTGATTT
GGTGGCTAAGCACCATCTGCAAGTGTTAGCCTCGCGTGACTTTGTGAAATTGCGTAAAGAACTAGGCTGTGATGAGGCTA
CCTTAAAGCAGGTGCAGCAACTAATTACCAGTTTAAATCCACGCCCTGGCAGTGCTTTTAGCCATATAGGTTCAGAGCAC
TATATCCAGCATGAAGTGATTGTGAAAAAGGTCAAAGGCATTTGGATTGCCAGCTTAAATGACAGTGTGATTCCTAAACT
GAAGATCAACCAGCTGTACGCTGGTATCTTGAAGCGCAATCGTGATAGTTCCAGTCAGTACCTGCAAAGTCAGATGCAGG
AAGCCAAATGGATGATTAAGAATATCCAGCAGCGTTTTTCTACCATTTTGCGTGTATCGCAGGCGATTGTAGATAGACAG
CGCAACTTTCTGGAGTATGGTGAAGTTGCCATGCGCCCGCTAGTATTAAGAGAGATTGCCGATGAGCTTGATTTGCACGA
GTCAACAATTTCACGCGTGACGACCAATAAATACATGCTGACACCGCGCGGTATTTTTGAACTTAAGTACTTTTTTGGCA
GTTCAGTGGCGACCGATGCTGGCGGCACTTGTTCGGCAACCGCCATTCGCGCCCTGATTAAGCAGTTGGTGGATCAAGAA
AATCCAAGGAAGCCATACTCGGATAATCAGATTACAGATTTGCTGGCTAAACAGGGTATTGTGGTCGCGCGGCGCACGAT
TGCCAAGTATCGTGAGTCTATGAATATCGCGCCAGCAAGCTTAAGAAAATCCTTATAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(161-353)

Sigma-54, DNA binding domain (Sigma54_DBD)

(366-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6WT04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.81

100

0.499

Multiple sequence alignment