Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII78_RS13865 Genome accession   NZ_CP045824
Coordinates   2648579..2648989 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain MB8_B10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2643579..2653989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII78_RS13835 (GII78_13835) - 2643691..2643831 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII78_RS13840 (GII78_13840) yqeF 2644137..2644868 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  GII78_RS13845 (GII78_13845) cwlH 2645120..2645872 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII78_RS13850 (GII78_13850) yqeD 2646059..2646685 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  GII78_RS13855 (GII78_13855) gnd 2646704..2647572 (-) 869 Protein_2704 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII78_RS13860 (GII78_13860) yqeB 2647824..2648546 (+) 723 WP_010886572.1 hypothetical protein -
  GII78_RS13865 (GII78_13865) nucA/comI 2648579..2648989 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII78_RS13870 (GII78_13870) - 2649185..2649532 (+) 348 Protein_2707 sigma-70 family RNA polymerase sigma factor -
  GII78_RS13875 (GII78_13875) spoIVCA 2649563..2651023 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  GII78_RS22270 - 2650981..2651159 (-) 179 Protein_2709 hypothetical protein -
  GII78_RS13880 (GII78_13880) arsC 2651514..2651933 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GII78_RS13885 (GII78_13885) acr3 2651945..2652985 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  GII78_RS13890 (GII78_13890) arsK 2653008..2653448 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GII78_RS13895 (GII78_13895) arsR 2653509..2653826 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=347748 GII78_RS13865 WP_009967785.1 2648579..2648989(-) (nucA/comI) [Bacillus subtilis strain MB8_B10]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=347748 GII78_RS13865 WP_009967785.1 2648579..2648989(-) (nucA/comI) [Bacillus subtilis strain MB8_B10]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529