Detailed information NTG0347714

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GII78_RS08550
• Genome accession: NZ_CP045824
• Coordinates: 1648250..1649239 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain MB8_B10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1643250..1654239

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII78_RS08535 (GII78_08535)	acpP	1643447..1643680 (+)	234	WP_003154310.1	acyl carrier protein	-
GII78_RS08540 (GII78_08540)	rncS	1643820..1644569 (+)	750	WP_003232030.1	ribonuclease III	-
GII78_RS08545 (GII78_08545)	smc	1644670..1648230 (+)	3561	WP_113712980.1	chromosome segregation protein SMC	-
GII78_RS08550 (GII78_08550)	pilA	1648250..1649239 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
GII78_RS08555 (GII78_08555)	sivC	1649276..1649761 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
GII78_RS08560 (GII78_08560)	srpM	1649938..1650270 (+)	333	WP_003238590.1	putative DNA-binding protein	-
GII78_RS08565 (GII78_08565)	ffh	1650284..1651624 (+)	1341	WP_003232020.1	signal recognition particle protein	-
GII78_RS08570 (GII78_08570)	rpsP	1651730..1652002 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
GII78_RS08575 (GII78_08575)	khpA	1652002..1652247 (+)	246	WP_014476792.1	KH domain-containing protein	-
GII78_RS08580 (GII78_08580)	ylqD	1652369..1652755 (+)	387	WP_014476793.1	YlqD family protein	-
GII78_RS08585 (GII78_08585)	rimM	1652760..1653284 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
GII78_RS08590 (GII78_08590)	trmD	1653281..1654012 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=347714 GII78_RS08550 WP_003232026.1 1648250..1649239(+) (pilA) [Bacillus subtilis strain MB8_B10]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=347714 GII78_RS08550 WP_003232026.1 1648250..1649239(+) (pilA) [Bacillus subtilis strain MB8_B10]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGTGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCAGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401