Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MMOL_RS03015 Genome accession   NC_012968
Coordinates   631289..633013 (-) Length   574 a.a.
NCBI ID   WP_015831542.1    Uniprot ID   -
Organism   Methylotenera mobilis JLW8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 626289..638013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMOL_RS02995 (Mmol_0591) - 626700..627863 (+) 1164 WP_015831538.1 FAD-dependent monooxygenase -
  MMOL_RS03000 (Mmol_0592) - 627860..629056 (+) 1197 WP_015831539.1 FAD-dependent monooxygenase -
  MMOL_RS03005 (Mmol_0593) - 629193..629909 (+) 717 WP_015831540.1 DsbC family protein -
  MMOL_RS03010 (Mmol_0594) pilC 629976..631199 (-) 1224 WP_015831541.1 type II secretion system F family protein Machinery gene
  MMOL_RS03015 (Mmol_0595) pilB 631289..633013 (-) 1725 WP_015831542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MMOL_RS03020 (Mmol_0596) - 633374..633577 (+) 204 WP_015831543.1 cold-shock protein -
  MMOL_RS03025 (Mmol_0597) icd 633688..634941 (-) 1254 WP_015831544.1 NADP-dependent isocitrate dehydrogenase -
  MMOL_RS03030 (Mmol_0598) - 634957..635511 (+) 555 WP_015831545.1 pseudouridine synthase -
  MMOL_RS03035 (Mmol_0599) - 635508..636218 (+) 711 WP_015831546.1 type 1 glutamine amidotransferase -
  MMOL_RS03040 (Mmol_0600) aroC 636414..637502 (+) 1089 WP_015831547.1 chorismate synthase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62863.08 Da        Isoelectric Point: 5.1137

>NTDB_id=34764 MMOL_RS03015 WP_015831542.1 631289..633013(-) (pilB) [Methylotenera mobilis JLW8]
MATATSQTKLSGLARTLIQEKLLSEDEARAVEAQANSANASFISQLIQSKKLSALSIAEASAKAFGFPYFNLDAFNPDYL
PAKQIDSKLVISNRVIGLQARNNVLYVAISDPTNILALDSVQFQMGMNISPIVVEDDKLGRWIEKVVQAKDTSLKSLAVG
NDDDFGFSDDNSQAAEEEQVETEVDDAPVVRFLNKMLLDAINMGASDLHFEPYEKFYRVRYRIDGALREINQPPLAIKEK
LASRIKVISSMDISEKRIPQDGRMKLVLSKTRTIDFRVSTLPLIHGEKIVMRILDPASATLGIEALGYEPIQKERLLNAV
SRPYGLVLVTGPTGSGKTVSLYTCLNILNSPDVNISTAEDPCEIPLAGINQVNVNDKQGLTFAAALKSFLRQDPDVIMIG
EIRDLETADMAIKAASTGHMVLSTLHTNDAPSTLTRLLNMGVAPFNIASAVSLITAQRLARRLCKHCKLPINVPKEALLN
VGFIEEDFNESWQLYGHNPEGCELCNGGYKGRVGIYQVMPITDAISRIIMKNGTAHDIADQAKIEGVNDLRRSGVLKVMQ
GLTSIEEVEACTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=34764 MMOL_RS03015 WP_015831542.1 631289..633013(-) (pilB) [Methylotenera mobilis JLW8]
ATGGCAACTGCAACTTCACAAACCAAACTAAGTGGTCTAGCTAGAACATTAATTCAAGAAAAGCTGCTTTCCGAAGATGA
AGCGAGGGCGGTGGAAGCTCAAGCTAATTCTGCAAATGCCTCATTTATTTCACAGCTGATACAAAGCAAAAAACTCAGCG
CATTAAGTATTGCGGAGGCCTCCGCCAAAGCCTTTGGTTTCCCATATTTTAATCTAGACGCTTTCAATCCAGACTATCTC
CCAGCCAAACAAATTGACAGCAAACTAGTTATTAGCAATCGTGTCATCGGTCTGCAAGCGCGCAACAACGTGCTCTATGT
CGCCATCTCCGATCCCACTAACATACTAGCGCTAGATAGCGTGCAATTCCAAATGGGGATGAACATTTCACCTATTGTGG
TGGAAGATGACAAACTTGGCCGCTGGATAGAAAAGGTGGTGCAAGCTAAAGATACCAGCCTTAAATCATTGGCTGTAGGC
AATGATGACGATTTTGGCTTTAGTGATGACAACTCACAAGCAGCAGAAGAGGAACAAGTCGAAACCGAAGTTGACGACGC
TCCTGTGGTACGCTTTCTTAACAAGATGCTACTAGATGCTATCAACATGGGGGCATCTGATTTACATTTTGAGCCTTATG
AGAAGTTTTACCGTGTACGCTACCGTATTGATGGCGCATTGCGAGAGATTAACCAACCCCCTCTCGCTATTAAAGAAAAA
CTCGCATCACGCATTAAAGTAATCTCAAGTATGGACATTTCGGAAAAACGTATTCCGCAAGATGGCCGTATGAAGTTAGT
CTTATCCAAGACACGCACCATTGACTTCCGTGTCAGCACACTGCCACTCATTCATGGCGAAAAAATCGTAATGCGTATTC
TCGACCCTGCCAGTGCCACACTTGGAATTGAGGCGCTGGGTTATGAACCTATACAAAAAGAGCGCCTACTAAATGCAGTG
AGCCGCCCCTACGGCTTAGTACTAGTAACCGGCCCAACAGGTTCTGGTAAGACCGTATCCCTATATACATGCCTCAATAT
TCTCAACAGCCCAGATGTAAACATATCTACAGCTGAAGACCCATGCGAGATTCCGCTGGCAGGGATTAATCAGGTTAACG
TGAATGACAAACAAGGCCTGACATTTGCCGCTGCGCTCAAGTCATTCTTACGACAAGATCCTGATGTCATTATGATTGGT
GAGATTCGCGATTTAGAAACTGCAGACATGGCGATCAAGGCAGCATCCACCGGCCATATGGTATTGTCCACACTACATAC
TAATGATGCTCCATCCACGTTAACGCGCTTGCTCAATATGGGTGTGGCACCATTCAACATTGCGTCAGCTGTCTCATTGA
TTACAGCACAGCGCTTGGCGCGACGTTTGTGCAAACACTGTAAACTCCCGATCAACGTGCCTAAAGAGGCGCTCTTGAAT
GTGGGCTTTATTGAAGAAGACTTCAATGAGAGCTGGCAACTTTATGGTCACAACCCAGAAGGTTGCGAACTATGCAACGG
CGGCTATAAAGGCCGTGTGGGCATTTACCAAGTGATGCCGATTACTGATGCCATTAGCCGCATTATCATGAAAAACGGCA
CTGCACATGATATTGCAGACCAAGCCAAAATCGAAGGCGTAAATGATTTACGTCGCTCTGGTGTGCTGAAAGTGATGCAA
GGGTTAACCTCTATAGAAGAAGTAGAAGCTTGTACCAATGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.167

100

0.544
  pilB Acinetobacter baumannii D1279779

54.073

100

0.544
  pilF Neisseria gonorrhoeae MS11

51.67

99.129

0.512
  pilB Legionella pneumophila strain ERS1305867

49.308

100

0.497
  pilB Vibrio cholerae strain A1552

47.1

99.129

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

44.742

97.735

0.437
  pilB Vibrio campbellii strain DS40M4

43.617

98.258

0.429
  pilF Thermus thermophilus HB27

38.85

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.435

100

0.385

Multiple sequence alignment