Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Pstu14405_RS18625 Genome accession   NZ_CP036186
Coordinates   4023436..4025508 (-) Length   690 a.a.
NCBI ID   WP_003283485.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain ATCC 14405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4018436..4030508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Pstu14405_RS18610 (Pstu14405_18715) - 4020179..4021747 (-) 1569 WP_003283489.1 AAA family ATPase -
  Pstu14405_RS18615 (Pstu14405_18720) aroB 4021758..4022861 (-) 1104 WP_003283487.1 3-dehydroquinate synthase -
  Pstu14405_RS18620 (Pstu14405_18725) aroK 4022885..4023430 (-) 546 WP_003283486.1 shikimate kinase AroK -
  Pstu14405_RS18625 (Pstu14405_18730) pilQ 4023436..4025508 (-) 2073 WP_003283485.1 type IV pilus secretin PilQ Machinery gene
  Pstu14405_RS18630 (Pstu14405_18735) pilP 4025562..4026089 (-) 528 WP_003283484.1 type 4a pilus biogenesis lipoprotein PilP -
  Pstu14405_RS18635 (Pstu14405_18740) pilO 4026086..4026709 (-) 624 WP_003283483.1 type 4a pilus biogenesis protein PilO -
  Pstu14405_RS18640 (Pstu14405_18745) pilN 4026706..4027278 (-) 573 WP_003283481.1 type 4a pilus biogenesis protein PilN -
  Pstu14405_RS18645 (Pstu14405_18750) comM 4027278..4028342 (-) 1065 WP_003283479.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 73849.42 Da        Isoelectric Point: 4.8282

>NTDB_id=347419 Pstu14405_RS18625 WP_003283485.1 4023436..4025508(-) (pilQ) [Stutzerimonas stutzeri strain ATCC 14405]
MNSTLTRLGVSLLAAFISPTLLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTTLVPYSTRVEGNNLFVVLGEGAGNRSSVVSVAAAAPAAASSVDSLKIGKTIENVD
FRRGDDGAGNVVITLSDPSVSPTIEEQGGRIRVVFAKTQLPESLRVRLDVQDFATPVKFVDASARAGSASIAIEPTGRYD
YLAYQTDNNLTVSVKPLTEDEADKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTSNTASAE
DRGSIAVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVNWRGARVGDNNFVIGGAGGNR
VGAGDNPATALGTFVDLGATGSTTGIGIGFITDNTILDLQLSAMEKTGNGEVVSQPKVVTADKETAKILKGAEVPYQEAS
SSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAASTGGVPSIRKNEVNAKVLVTDGETIVIGGVFSNTQ
SKSVDKVPFLGDVPFVGRLFRRDLVQDRKSELLVFITPRIMNNQAIQAGR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=347419 Pstu14405_RS18625 WP_003283485.1 4023436..4025508(-) (pilQ) [Stutzerimonas stutzeri strain ATCC 14405]
ATGAACAGTACCCTTACACGCCTGGGCGTTTCTCTGCTGGCGGCGTTTATTTCACCGACGCTCTTGGCGGCCAATTTAAA
CGCGCTAGACGTTGCTGCCCTGCCTGGCGATCGTGTCGAGTTGAAGTTGGCATTCGATGAGCCTGTGGCATCGCCGCGTG
GTTATACGCTGGACCAGCCGGCGCGCATTGCGCTCGATCTTCCTGGTGTTTCTAATAATTTGGGTGCGAAGAATCGCGAG
CTCGGGGTTGGTAATGCTCGGAGCGTGACTGTGGTGGAGGCCCAGGGACGTACACGTCTGATCGTTAACCTGACCACGCT
TGTACCTTACTCGACTCGAGTGGAAGGCAATAATCTGTTCGTAGTGTTAGGTGAAGGAGCTGGAAACCGCTCGTCGGTCG
TTTCAGTCGCCGCGGCGGCCCCTGCCGCCGCATCGTCAGTGGATTCGCTTAAGATTGGTAAAACTATTGAGAACGTTGAT
TTCCGTCGCGGGGACGACGGGGCCGGAAACGTGGTCATCACGCTGTCCGATCCATCGGTCAGTCCTACTATTGAGGAGCA
AGGAGGGAGGATCAGGGTTGTATTCGCTAAGACCCAGTTACCAGAATCGCTACGAGTTCGTCTCGACGTTCAGGACTTTG
CAACGCCTGTAAAATTCGTTGATGCCTCCGCGCGCGCGGGTAGTGCGAGCATCGCCATTGAGCCGACTGGTCGGTATGAC
TATCTGGCCTACCAAACTGACAACAACCTTACTGTCAGCGTTAAGCCTCTAACGGAAGATGAAGCGGATAAGCGCAAAGC
CGACAGATTCGCCTATTCGGGCGAGAAACTCTCGCTCAACTTTCAGGATATTGATGTTCGCTCGGTGCTTCAGCTGATCG
CTGACTTTACTGATCTGAATCTTGTTGCGAGTGATACGGTGAGCGGCAATATCACTTTGCGTTTGCAGAACGTGCCGTGG
GATCAAGCCCTGGATTTGGTTTTAAAAACCAAGGGTCTGGATAAGCGTCAAGTGGGTAACGTGCTACTCGTTGCGCCGGC
AGACGAGATTGCGGCCCGTGAGCGGCAAGAACTGGAGTCCCAGCGTCAGATTGCTGAACTTGCTCCGCTACGTCGGGAGG
TTGTGCAGGTCAATTACGCCAAGGCGGCTGACATTGCAAGGTTATTCCAGTCCGTGACCAGTAACACCGCTTCGGCAGAA
GACCGTGGATCCATCGCAGTGGATGATCGTACAAACAACATCATCGCTTATCAAACGCAAGATCGGCTTGATGAGCTCCG
CCGCATCGTTGCCCAGCTTGACATCCCTGTTCGTCAGGTAATGATTGAGGCACGAATTGTAGAAGCTAACGTCGATTACG
ATAAAAGCCTTGGCGTTAACTGGCGTGGCGCTAGAGTCGGCGACAATAATTTTGTCATAGGCGGAGCTGGTGGGAACCGC
GTAGGTGCTGGCGATAATCCAGCTACCGCGCTTGGTACATTTGTGGACCTTGGTGCGACGGGATCTACTACCGGTATTGG
TATCGGTTTTATAACCGATAACACTATTTTGGATCTGCAGCTTTCGGCAATGGAGAAAACTGGTAATGGGGAGGTGGTAT
CACAACCTAAAGTTGTGACTGCAGATAAAGAAACCGCAAAGATCTTGAAGGGGGCTGAAGTTCCTTATCAAGAGGCTAGT
TCCAGTGGGGCGACTAGCACCTCCTTCAAGGAGGCCGCCCTCTCTCTTGAGGTGACGCCTCAGATTACTCCGGACAACCG
GATTATAATGGAAGTGAAAGTAACAAAGGATGAACCCGATTTTTCGCAGGCGGCTAGCACTGGCGGTGTACCTTCTATTC
GTAAAAATGAGGTTAACGCCAAGGTTTTGGTTACCGATGGTGAAACAATCGTGATTGGCGGCGTGTTCTCCAATACGCAG
AGCAAGTCGGTAGACAAAGTGCCCTTCCTTGGTGACGTACCGTTCGTTGGCCGTCTATTCCGCCGTGATCTGGTGCAAGA
TCGAAAGTCGGAGCTCCTTGTATTCATCACTCCACGGATCATGAATAATCAGGCGATTCAAGCCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(300-346)

AMIN domain (AMIN)

(38-123)

Bacterial type II and III secretion system protein (Secretin)

(523-681)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.501

100

0.764
  comQ Acinetobacter baylyi ADP1

41.364

99.855

0.413
  pilQ Acinetobacter baumannii strain A118

39.858

100

0.407
  pilQ Acinetobacter baumannii D1279779

39.858

100

0.407
  pilQ Legionella pneumophila strain Lp02

37.649

97.391

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.612

97.101

0.365

Multiple sequence alignment