Detailed information    

insolico Bioinformatically predicted

Overview

Name   helD   Type   Machinery gene
Locus tag   GII81_RS18235 Genome accession   NZ_CP045820
Coordinates   3479866..3482190 (-) Length   774 a.a.
NCBI ID   WP_029725920.1    Uniprot ID   -
Organism   Bacillus subtilis strain MB9_B1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3474866..3487190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII81_RS18225 (GII81_18225) cysI 3476137..3477852 (-) 1716 WP_029725918.1 assimilatory sulfite reductase (NADPH) hemoprotein subunit -
  GII81_RS18230 (GII81_18230) cysJ 3477878..3479695 (-) 1818 WP_029725919.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  GII81_RS18235 (GII81_18235) helD 3479866..3482190 (-) 2325 WP_029725920.1 DNA helicase IV Machinery gene
  GII81_RS18240 (GII81_18240) - 3482766..3483200 (+) 435 WP_046663943.1 hypothetical protein -
  GII81_RS18245 (GII81_18245) yvgT 3483304..3483915 (-) 612 WP_003228419.1 trimeric intracellular cation channel family protein -
  GII81_RS18250 (GII81_18250) bdbC 3484097..3484513 (-) 417 WP_003228417.1 disulfide oxidoreductase -
  GII81_RS18255 (GII81_18255) bdbD 3484518..3485186 (-) 669 WP_003228414.1 disulfide bond formation protein BdbD -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 89959.61 Da        Isoelectric Point: 6.4475

>NTDB_id=347313 GII81_RS18235 WP_029725920.1 3479866..3482190(-) (helD) [Bacillus subtilis strain MB9_B1]
MNQQDKEWKEEQSRIDEVLKELEKKERFLETSAGGLKHDIIGLRKSFWEDVKVNFDDAHEAIETMASIKQQAELLSDREQ
NHRRMDQQLKRIHQLKKSPYFGRIDFIENGEAEAERIYIGLASCLDEKEEHFLIYDWRAPISSLYYNYSPGKAEYEVPGE
TIEGEMVLKRQFMIKNGTLKAMFNTDMTIGDEMLQEVLSHHSDTQMKNIVSTIQKEQNQIIRNEKSKILFVQGAAGSGKT
SAALQRVAYLLYRHRGVIDAGQIVLFSPNFLFNSYVSSVLPELGEENMEQATFQEYIEHRLGRKFKCESPFDQLEYCLTE
TKDGDFPTRLAGITWKAGLSFQQFIDEYVTRLSSEGMIFKNIIFRGQKLITKEQIQSYFYSLDQNHSIPNRMEQTAKWLL
SELNKLEKKERRKDWVVHEAELLDKEDYLDVYKKLQERKRFSESTFNDYQREQQLLAAIIVKKAFKPLKQAVRLLAFLDV
SQLYLQLFSGWGGKFQHEKMAVIGELTRSAFTDNKLLYEDAAPFLYMQDLIEGRKKNTKIKHLFIDEAQDYSPFQMAYMR
SIFPAASMTVLGDINQSIYAHTIHGDQRMDACFEDDPAEYVRLKRTYRSTRQIVEFTKAMLQDGADIEPFNRSGEMPLVV
KTEGHESLCQKLAQEIGRLKKKGRETIAVICKTAQQCIQAHAHMSEYTDVRLIHKENQPFQKGVCVIPVYLAKGIEFDAV
LVYDASEEHYHTEHDRRLLYTACTRAMHMLAVFYTGEASPFVTAVPPHLYQMAE

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=347313 GII81_RS18235 WP_029725920.1 3479866..3482190(-) (helD) [Bacillus subtilis strain MB9_B1]
ATGAATCAGCAGGATAAGGAATGGAAGGAAGAGCAGTCGAGAATAGATGAGGTGCTGAAGGAGCTCGAAAAAAAAGAGCG
GTTTCTGGAAACGTCTGCGGGCGGGTTGAAGCATGATATCATCGGATTAAGAAAAAGCTTTTGGGAGGATGTTAAGGTTA
ATTTTGATGATGCCCATGAAGCGATTGAAACGATGGCAAGCATTAAGCAGCAGGCAGAGCTGCTGTCTGATCGGGAACAG
AATCATCGGCGCATGGATCAGCAGCTCAAACGAATTCATCAATTGAAAAAATCGCCTTATTTCGGCCGGATTGATTTTAT
TGAAAACGGCGAGGCGGAGGCAGAGCGAATTTATATCGGTTTAGCTTCCTGTTTGGATGAGAAAGAGGAACACTTTCTGA
TCTATGACTGGAGGGCGCCGATATCAAGTTTGTACTACAATTATTCTCCAGGAAAAGCGGAGTACGAGGTGCCCGGTGAA
ACGATAGAAGGGGAAATGGTGCTGAAACGCCAGTTTATGATCAAAAACGGCACTCTCAAGGCGATGTTTAATACCGATAT
GACGATTGGCGATGAAATGCTGCAAGAGGTATTAAGCCATCACTCCGATACCCAAATGAAAAACATCGTGTCTACGATTC
AGAAAGAACAAAATCAGATTATCCGTAATGAAAAAAGCAAGATCCTGTTCGTGCAGGGAGCGGCTGGCAGTGGGAAAACA
TCGGCTGCGCTTCAGCGTGTCGCTTATTTGTTATACAGGCACCGCGGCGTCATTGATGCCGGACAAATCGTTTTGTTTTC
TCCCAACTTCTTATTTAACAGCTATGTATCCTCTGTTTTGCCTGAGTTAGGAGAAGAGAATATGGAGCAGGCGACGTTTC
AGGAATATATCGAGCATCGTCTTGGCCGCAAGTTCAAATGTGAAAGCCCCTTTGATCAGCTCGAATATTGCCTGACGGAA
ACAAAGGACGGCGATTTTCCAACAAGGCTTGCGGGAATCACATGGAAGGCGGGTTTATCCTTTCAGCAATTCATTGATGA
ATACGTCACACGGCTTTCTTCTGAAGGGATGATTTTTAAAAATATCATATTCCGCGGACAAAAGCTGATCACAAAGGAGC
AAATTCAATCCTATTTTTACTCATTGGATCAAAATCACTCCATTCCGAATCGGATGGAGCAAACCGCGAAGTGGCTGCTG
AGTGAGCTTAACAAGCTTGAGAAAAAGGAACGGCGAAAGGATTGGGTTGTTCATGAAGCTGAGCTGCTTGACAAAGAGGA
CTACCTGGACGTGTACAAAAAACTGCAGGAAAGGAAACGGTTCAGCGAGAGTACGTTTAACGATTATCAAAGAGAGCAGC
AGCTGCTTGCCGCCATCATTGTGAAAAAAGCTTTCAAACCGCTCAAGCAGGCAGTCAGGCTCCTTGCATTTCTTGATGTT
TCACAGCTGTATTTGCAATTGTTTTCTGGCTGGGGAGGGAAATTTCAGCATGAGAAGATGGCCGTGATAGGTGAGCTGAC
CCGTTCGGCATTTACGGATAATAAATTGCTCTATGAGGACGCGGCACCGTTTCTTTATATGCAGGATCTGATTGAGGGCA
GAAAGAAAAATACGAAGATCAAACATCTATTTATTGACGAAGCCCAGGACTATTCACCGTTTCAGATGGCTTACATGAGA
AGTATTTTTCCGGCTGCCAGCATGACAGTGCTCGGTGATATCAATCAGTCCATCTACGCGCATACCATACACGGGGATCA
GCGTATGGATGCGTGCTTTGAGGATGATCCTGCTGAGTATGTTCGTTTAAAGAGAACATACAGATCAACAAGGCAGATTG
TCGAGTTTACCAAAGCGATGCTTCAAGATGGAGCGGATATTGAGCCGTTTAATAGAAGCGGGGAAATGCCGCTCGTTGTC
AAAACTGAAGGACATGAGAGCCTTTGCCAAAAGCTCGCGCAAGAAATTGGCCGGCTCAAGAAAAAAGGGCGCGAAACAAT
AGCCGTCATCTGCAAGACTGCGCAGCAATGCATACAGGCGCACGCCCACATGAGTGAATATACAGATGTTCGCCTGATTC
ATAAAGAAAATCAACCATTTCAAAAGGGTGTTTGCGTGATTCCGGTGTATTTAGCGAAGGGAATTGAATTTGACGCAGTG
CTTGTCTATGATGCATCTGAAGAACACTATCATACAGAGCACGACCGCAGGCTTTTATATACTGCGTGCACCCGTGCGAT
GCACATGCTGGCAGTATTTTATACAGGTGAAGCCAGTCCGTTTGTAACCGCTGTGCCGCCTCACTTATATCAGATGGCTG
AATGA

Domains

Predicted by InterProScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(704-751)

AAA domain (AAA_19)

(217-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  helD Bacillus subtilis subsp. subtilis str. 168

98.191

100

0.982

Multiple sequence alignment