Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KAN02_RS16370 Genome accession   NZ_CP036171
Coordinates   3404868..3405668 (-) Length   266 a.a.
NCBI ID   WP_004710659.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain KAN02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3399868..3410668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN02_RS16350 (KAN02_16350) - 3400933..3401631 (-) 699 WP_002053293.1 YfgM family protein -
  KAN02_RS16355 (KAN02_16355) hisS 3401660..3402952 (-) 1293 WP_087606378.1 histidine--tRNA ligase -
  KAN02_RS16360 (KAN02_16360) ispG 3402949..3404064 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KAN02_RS16365 (KAN02_16365) - 3404086..3404877 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  KAN02_RS16370 (KAN02_16370) pilF 3404868..3405668 (-) 801 WP_004710659.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KAN02_RS16375 (KAN02_16375) rlmN 3405684..3406916 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KAN02_RS16380 (KAN02_16380) ndk 3407039..3407470 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  KAN02_RS16385 (KAN02_16385) iscX 3407584..3407784 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  KAN02_RS16390 (KAN02_16390) - 3407884..3408435 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  KAN02_RS16395 (KAN02_16395) - 3408460..3409758 (-) 1299 WP_087606377.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.44 Da        Isoelectric Point: 9.2307

>NTDB_id=347301 KAN02_RS16370 WP_004710659.1 3404868..3405668(-) (pilF) [Acinetobacter nosocomialis strain KAN02]
MSTPKLKIMLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=347301 KAN02_RS16370 WP_004710659.1 3404868..3405668(-) (pilF) [Acinetobacter nosocomialis strain KAN02]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCC
AACGCTTAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCCGCTGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAGCGTTCCCTCGACCAAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCCGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.496

100

0.985
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment