Detailed information NTG0347251

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GII81_RS08500
• Genome accession: NZ_CP045820
• Coordinates: 1637765..1638754 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain MB9_B1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1632765..1643754

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII81_RS08485 (GII81_08485)	acpP	1632962..1633195 (+)	234	WP_003154310.1	acyl carrier protein	-
GII81_RS08490 (GII81_08490)	rncS	1633335..1634084 (+)	750	WP_003232030.1	ribonuclease III	-
GII81_RS08495 (GII81_08495)	smc	1634185..1637745 (+)	3561	WP_015715874.1	chromosome segregation protein SMC	-
GII81_RS08500 (GII81_08500)	pilA	1637765..1638754 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
GII81_RS08505 (GII81_08505)	sivC	1638791..1639276 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
GII81_RS08510 (GII81_08510)	srpM	1639453..1639785 (+)	333	WP_003238590.1	putative DNA-binding protein	-
GII81_RS08515 (GII81_08515)	ffh	1639799..1641139 (+)	1341	WP_003232020.1	signal recognition particle protein	-
GII81_RS08520 (GII81_08520)	rpsP	1641245..1641517 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
GII81_RS08525 (GII81_08525)	khpA	1641517..1641762 (+)	246	WP_014476792.1	KH domain-containing protein	-
GII81_RS08530 (GII81_08530)	ylqD	1641884..1642270 (+)	387	WP_014476793.1	YlqD family protein	-
GII81_RS08535 (GII81_08535)	rimM	1642275..1642799 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
GII81_RS08540 (GII81_08540)	trmD	1642796..1643527 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=347251 GII81_RS08500 WP_003232026.1 1637765..1638754(+) (pilA) [Bacillus subtilis strain MB9_B1]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=347251 GII81_RS08500 WP_003232026.1 1637765..1638754(+) (pilA) [Bacillus subtilis strain MB9_B1]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGTGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCAGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401