Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EXW42_RS04085 Genome accession   NZ_CP036137
Coordinates   783789..785165 (+) Length   458 a.a.
NCBI ID   WP_002169331.1    Uniprot ID   R8NPL5
Organism   Bacillus mycoides strain JAS85/1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 778789..790165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW42_RS04065 (EXW42_04160) ctsR 778988..779449 (+) 462 WP_002029353.1 transcriptional regulator CtsR -
  EXW42_RS04070 (EXW42_04165) - 779619..780167 (+) 549 WP_002169333.1 UvrB/UvrC motif-containing protein -
  EXW42_RS04075 (EXW42_04170) - 780172..781236 (+) 1065 WP_002169332.1 protein arginine kinase -
  EXW42_RS04080 (EXW42_04175) clpC 781259..783694 (+) 2436 WP_002063405.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EXW42_RS04085 (EXW42_04180) radA 783789..785165 (+) 1377 WP_002169331.1 DNA repair protein RadA Machinery gene
  EXW42_RS04090 (EXW42_04185) disA 785169..786242 (+) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  EXW42_RS04095 (EXW42_04190) - 786403..787506 (+) 1104 WP_002063406.1 PIN/TRAM domain-containing protein -
  EXW42_RS04100 (EXW42_04195) ispD 787523..788203 (+) 681 WP_002009811.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EXW42_RS04105 (EXW42_04200) ispF 788317..788793 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49854.60 Da        Isoelectric Point: 7.4250

>NTDB_id=347067 EXW42_RS04085 WP_002169331.1 783789..785165(+) (radA) [Bacillus mycoides strain JAS85/1]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSLYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVTQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=347067 EXW42_RS04085 WP_002169331.1 783789..785165(+) (radA) [Bacillus mycoides strain JAS85/1]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTTATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TCAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGAGAATTGCA
GCGCACATTGAGGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATCCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAACGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGGCCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TCGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAGCCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8NPL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment