Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EXW56_RS05740 Genome accession   NZ_CP036132
Coordinates   1140703..1144428 (+) Length   1241 a.a.
NCBI ID   WP_215597297.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS823     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1135703..1149428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW56_RS05725 (EXW56_05845) - 1135861..1136454 (+) 594 WP_002201415.1 TVP38/TMEM64 family protein -
  EXW56_RS05730 (EXW56_05850) lepB 1136510..1137073 (+) 564 WP_002158924.1 signal peptidase I -
  EXW56_RS05735 (EXW56_05855) addB 1137191..1140706 (+) 3516 WP_215597296.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EXW56_RS05740 (EXW56_05860) addA 1140703..1144428 (+) 3726 WP_215597297.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EXW56_RS05745 (EXW56_05865) - 1144452..1144730 (+) 279 WP_002201412.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  EXW56_RS05750 (EXW56_05870) - 1144947..1145654 (+) 708 WP_002201411.1 DNA alkylation repair protein -
  EXW56_RS05755 (EXW56_05875) - 1145924..1146409 (+) 486 WP_002201410.1 hypothetical protein -
  EXW56_RS05760 (EXW56_05880) gerPF 1146495..1146710 (-) 216 WP_001141570.1 spore germination protein GerPF -
  EXW56_RS05765 (EXW56_05885) - 1146753..1147139 (-) 387 WP_002140807.1 spore germination protein GerPE -
  EXW56_RS05770 (EXW56_05890) gerPD 1147154..1147348 (-) 195 WP_001052805.1 spore germination protein GerPD -
  EXW56_RS05775 (EXW56_05895) - 1147355..1147969 (-) 615 WP_002158914.1 spore germination protein GerPC -
  EXW56_RS05780 (EXW56_05900) gerPB 1148034..1148240 (-) 207 WP_002201409.1 spore germination protein GerPB -
  EXW56_RS05785 (EXW56_05905) gerPA 1148255..1148476 (-) 222 WP_002201408.1 spore germination protein GerPA -
  EXW56_RS05790 (EXW56_05910) - 1148573..1148752 (-) 180 WP_002087032.1 aspartyl-phosphate phosphatase Spo0E family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142606.76 Da        Isoelectric Point: 4.8528

>NTDB_id=347017 EXW56_RS05740 WP_215597297.1 1140703..1144428(+) (addA) [Bacillus mycoides strain JAS823]
MIENWPEKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDGPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEEHI
RKATELAMLPDGPAPRAETLQADLALLGTLSSAARGSWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVKLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYKLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPGEIYGYSASWK
VEVVDGKTLLAPEPVQEEKQELLEALRDKKAVPLESERKEEVYDRLMWEYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIARMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQGKSDESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPTL
EDRYKVQLSLYAKALEKSLKHPVKEKCLYFFDGNHVVNIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=347017 EXW56_RS05740 WP_215597297.1 1140703..1144428(+) (addA) [Bacillus mycoides strain JAS823]
ATGATAGAAAATTGGCCTGAGAAACCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAAATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTATTAATTGATGGGCCAGGTTCACAGCATATAAGAAAACAGCTTAGCTTATTAAATAAAGC
TTCCATTTCTACCATTCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTCGATCCTCGTTTTC
GTATTGCGAACCAAACAGAAAATGAGTTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAATATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGTTATACGAGTGACCGTAGTGACGATGACTTACAACGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAGAAGTGGCTTGATAAATTAGTAGAAGCATACGATGTTGAAGGAA
AGACGATTGAAGATTTGGTTTACGCTTCTTACCTATTAGAAGATGTAAAGTTTCAGCTTGAAACAGCGGAAGAGCATATT
CGTAAGGCGACTGAACTCGCAATGCTTCCTGATGGTCCAGCGCCTCGTGCCGAAACTTTACAAGCGGATTTAGCTTTACT
TGGAACGTTATCATCAGCAGCTCGTGGATCGTGGACAAGCGTTTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGATTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTGAAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATTAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGCGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTAAAACAGTCAATCTATCGTTTCCGACTAGCAG
AGCCTGGTTTATTCTTAGGAAAATATAAACGTTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGCCGTCATGAAGTGCTAGCTGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGATTATGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACTGAGGAAGAAGTGCAAGAAGGTGAAGAAGGTACAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGCTATGAAGTGTATGATCGTAAAACTGATAGTATGCGCCCGGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCTGTACTTCGTTCACCGATCGTTGGACTAAATGACGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCGCCGCTTGAAGAAGAACAAGAAC
TGCATGATAAATTAGAGTGGTTTTATAAATTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAATCTCTTTCCGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGACTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGACAGGCAAACTT
ACGCGTATTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGATTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGACATGGGAACGGCGAGAGCTCTTGGAGAACAAGAAGACGTCGTTCGCATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCAGTCGTATTTGTAGCTGGACTTGGTCGCCGTTTTAATACACAAGATTTAATGAA
GCGTTTCTTACTTCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCGATTAAGCGTAAAATGAAGATGGAATTAATTGCGGAGGAAATGCGCGTATTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCATGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGGAATATTCCAGGCGAAATTTATGGGTATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCAGTTCAAGAAGAGAAACAAGAGTTATTAGAAGCGCTTCG
CGATAAAAAAGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGGAATATGGGTATGAGG
ACGCGACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTTCGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGCTAACATACGCAGAGCGAGG
GACAGCAGTTCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAACAAATTGCAA
GAATGGTAAATAAAGAACTATTAACATTTGAACAAGCTGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCAGCAAAAAGTGTTGAACGTGAAGTACCATTTACGATGATGCTTTCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGTAAGAGTGACGAATCGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GAATTACTTTAATCGACTTTAAGACAGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAACCAACTTTA
GAAGACCGATACAAAGTACAGCTTTCACTATATGCAAAAGCACTCGAGAAAAGCTTAAAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.729

100

0.54

Multiple sequence alignment