Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   EXW56_RS00020 Genome accession   NZ_CP036132
Coordinates   3023..4150 (+) Length   375 a.a.
NCBI ID   WP_002113239.1    Uniprot ID   J7YA25
Organism   Bacillus mycoides strain JAS823     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW56_RS00005 (EXW56_00005) dnaA 1..1341 (+) 1341 WP_002016481.1 chromosomal replication initiator protein DnaA -
  EXW56_RS00010 (EXW56_00010) dnaN 1524..2669 (+) 1146 WP_002113238.1 DNA polymerase III subunit beta -
  EXW56_RS00015 (EXW56_00015) yaaA 2798..3010 (+) 213 WP_002016484.1 S4 domain-containing protein YaaA -
  EXW56_RS00020 (EXW56_00020) recF 3023..4150 (+) 1128 WP_002113239.1 DNA replication/repair protein RecF Machinery gene
  EXW56_RS00025 (EXW56_00025) gyrB 4189..6111 (+) 1923 WP_033714071.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  EXW56_RS00030 (EXW56_00030) gyrA 6200..8674 (+) 2475 WP_002113241.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 43500.76 Da        Isoelectric Point: 6.8417

>NTDB_id=347006 EXW56_RS00020 WP_002113239.1 3023..4150(+) (recF) [Bacillus mycoides strain JAS823]
MFITEIQLKNYRNYEHLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDYGNIKGRLQRRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGEMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGAKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLLGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=347006 EXW56_RS00020 WP_002113239.1 3023..4150(+) (recF) [Bacillus mycoides strain JAS823]
TTGTTTATTACAGAAATACAATTAAAAAACTATCGCAATTATGAGCATTTGGAGCTTTCCTTTGAGGATAAAGTCAATGT
AATTATTGGTGAAAATGCGCAAGGGAAAACGAATCTGATGGAAGCTATTTATGTATTGGCGATGGCTAAATCCCATAGAA
CTTCGAACGATCGTGAACTCATTCGTTGGGATGAGGATTATGGTAATATAAAAGGTAGATTACAAAGGCGAAATAGCTCA
TTATCCTTAGAATTAAATATTTCAAAAAAAGGTAAAAAGGCAAAGTTAAATCAGTTAGAACAACAAAAATTGAGCCAGTA
TATTGGTGAAATGAATGTTGTTATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTACGAAGACGCT
TTTTGGATATGGAACTTGGACAAATAGCTCCAGTCTATTTGTATGAATTAAGTCAATACCAAAAGGTACTCACGCAACGA
AATCATTTACTTAAGAAGATGCAAGGGAATAGTAAAAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTGCAAAAATTTTGCGGAAACGTTTTGAATTTTTGCATTTACTACAAGAATGGGCTGCTCCAATTCATCGCGGAA
TTAGCAGGGGATTAGAGGAGTTAGAAATCGTTTATAAACCAAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCTGTGAAACAACGTGAAATTTTCCGTGGTACAACTTTACTCGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCACAAGGACAACAACGAACAACCGCACTAT
CCCTGAAATTGGCTGAAATTGAATTGATATACTCAGAGGTTAAGGAATATCCAATCCTTTTACTAGATGATGTGTTATCA
GAATTAGATGATTATCGCCAATCGCATCTGCTAAATACAATTCAAGGAAAAGTACAAACCTTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTGAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGACA
GGAAATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J7YA25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

67.12

98.133

0.659

Multiple sequence alignment