Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   EXW25_RS21690 Genome accession   NZ_CP036111
Coordinates   4258595..4259341 (-) Length   248 a.a.
NCBI ID   WP_000487005.1    Uniprot ID   A0A2B5BXL0
Organism   Bacillus toyonensis strain JAS411     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4253595..4264341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW25_RS21670 (EXW25_22105) dnaG 4254355..4256151 (-) 1797 WP_000528230.1 DNA primase -
  EXW25_RS21675 (EXW25_22110) - 4256405..4256644 (-) 240 WP_002044942.1 DUF188 domain-containing protein -
  EXW25_RS21680 (EXW25_22115) - 4257013..4257825 (-) 813 WP_000368950.1 pyruvate, water dikinase regulatory protein -
  EXW25_RS21685 (EXW25_22120) - 4257855..4258487 (-) 633 WP_000583755.1 helix-turn-helix transcriptional regulator -
  EXW25_RS21690 (EXW25_22125) recO 4258595..4259341 (-) 747 WP_000487005.1 DNA repair protein RecO Machinery gene
  EXW25_RS21695 (EXW25_22130) - 4259375..4259518 (-) 144 WP_000883923.1 YqzL family protein -
  EXW25_RS21700 (EXW25_22135) era 4259691..4260596 (-) 906 WP_001080234.1 GTPase Era -
  EXW25_RS21705 (EXW25_22140) - 4260589..4260987 (-) 399 WP_001086291.1 cytidine deaminase -
  EXW25_RS21710 (EXW25_22145) - 4261086..4261439 (-) 354 WP_000715513.1 diacylglycerol kinase family protein -
  EXW25_RS21715 (EXW25_22150) ybeY 4261436..4261906 (-) 471 WP_097952932.1 rRNA maturation RNase YbeY -
  EXW25_RS21720 (EXW25_22155) - 4261903..4264047 (-) 2145 WP_000091505.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28620.17 Da        Isoelectric Point: 8.0566

>NTDB_id=346890 EXW25_RS21690 WP_000487005.1 4258595..4259341(-) (recO) [Bacillus toyonensis strain JAS411]
MFQKVEGIVIRTTDYGETNKIVTIFSREFGKISAMARGAKKPKSRLASVSQLMTHGHFLIQVGSGLGTLQQGEIISTMKE
IREDIFLTAYASFVVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=346890 EXW25_RS21690 WP_000487005.1 4258595..4259341(-) (recO) [Bacillus toyonensis strain JAS411]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAATAAGATTGTTACAATATTTTCAAG
AGAATTCGGTAAGATAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGGCATTTTCTTATTCAAGTGGGATCTGGGCTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTGTTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTACTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCTGTATTAGGGATGCGCCCGTATTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCGGAGCAAGATCCGTATCGTATACCGGTGGG
AGAAGCTGTACATAAATTGTTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGCTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTCCTA
GAGCAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B5BXL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

56.275

99.597

0.56

Multiple sequence alignment