Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EXW25_RS21415 Genome accession   NZ_CP036111
Coordinates   4205135..4206178 (-) Length   347 a.a.
NCBI ID   WP_001013173.1    Uniprot ID   -
Organism   Bacillus toyonensis strain JAS411     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4200135..4211178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW25_RS30780 - 4200192..4200296 (+) 105 Protein_4202 IS3 family transposase -
  EXW25_RS21370 (EXW25_21805) - 4200560..4200760 (-) 201 WP_000106086.1 YqzE family protein -
  EXW25_RS21375 (EXW25_21810) - 4200799..4201296 (-) 498 WP_097952966.1 shikimate kinase -
  EXW25_RS21380 (EXW25_21815) - 4201417..4202067 (-) 651 WP_098911397.1 2OG-Fe(II) oxygenase -
  EXW25_RS21385 (EXW25_21820) comGG 4202242..4202613 (-) 372 WP_000743703.1 competence type IV pilus minor pilin ComGG -
  EXW25_RS21390 (EXW25_21825) comGF 4202610..4203083 (-) 474 WP_000477948.1 competence type IV pilus minor pilin ComGF -
  EXW25_RS21395 (EXW25_21830) - 4203053..4203355 (-) 303 WP_141499334.1 competence protein ComG -
  EXW25_RS21400 (EXW25_21835) comGD 4203348..4203803 (-) 456 WP_097952962.1 competence type IV pilus minor pilin ComGD -
  EXW25_RS21405 (EXW25_21840) comGC 4203800..4204099 (-) 300 WP_001178691.1 competence type IV pilus major pilin ComGC -
  EXW25_RS21410 (EXW25_21845) comGB 4204111..4205148 (-) 1038 WP_275952053.1 competence type IV pilus assembly protein ComGB -
  EXW25_RS21415 (EXW25_21850) comGA 4205135..4206178 (-) 1044 WP_001013173.1 competence type IV pilus ATPase ComGA Machinery gene
  EXW25_RS21420 (EXW25_21855) - 4206383..4207078 (+) 696 WP_000434114.1 helix-turn-helix domain-containing protein -
  EXW25_RS21425 (EXW25_21860) - 4207204..4207446 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  EXW25_RS21430 (EXW25_21865) - 4207554..4208948 (+) 1395 WP_001094345.1 L-cystine transporter -
  EXW25_RS21435 (EXW25_21870) - 4209011..4209403 (-) 393 WP_097952958.1 hypothetical protein -
  EXW25_RS21440 (EXW25_21875) - 4209491..4209706 (-) 216 WP_001008324.1 DUF3912 family protein -
  EXW25_RS21445 (EXW25_21880) - 4209959..4210270 (+) 312 WP_001092835.1 hypothetical protein -
  EXW25_RS21450 (EXW25_21885) - 4210306..4210794 (-) 489 WP_000764243.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39220.57 Da        Isoelectric Point: 8.5491

>NTDB_id=346889 EXW25_RS21415 WP_001013173.1 4205135..4206178(-) (comGA) [Bacillus toyonensis strain JAS411]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLITKQCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYRESLVIRLHLQASVQPLSHLSLFPSTAEKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKRKTRRIVTLEDPVEKRKDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSILCKSMRQVRQASVYELLDGYDLQQAIKEANGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=346889 EXW25_RS21415 WP_001013173.1 4205135..4206178(-) (comGA) [Bacillus toyonensis strain JAS411]
ATGAATGGAATTGAAATTTTTGCGAATACAATTTTGAAAGAAGCGTGTAGGGTACAAGCATCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAGCTACGTATAGGAAAAGATTTAATTACGAAACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTTTCGCACTTTAAATTTTTGGCATCCATGGATATAGGAGAGAGGCGGAAGCCACAAAATGGTTCATTGTAT
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCGACTGTATATCGAGAAAGTCTCGTGATTCGCCT
TCATTTACAAGCATCTGTGCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACGGCGGAAAAACTCCTCTCTTTTTTAC
GTTATTCACATGGTCTACTCGTATTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAGGAAAACACGTCGAATTGTAACACTTGAAGACCCAGTTGAAAAAAGAAAAGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTACGCCATGATCCAGATATTATATTAGTTG
GTGAAATCCGAGATGAAGAAACAGCAAAAATAGCTGTAAGAGCAAGTTTAACTGGACATTTAGTAATGACGACCCTGCAT
ACGAATGATGCGAAAGGAGCGATACTCAGATTTATGGATTATGGCATAACGAGGCAAGAAATTGAACAATCTTTATTGGC
TGTAGCTGCACAGCGACTTGTTGAATTAAAGTGTCCATTTTGCAGAGGAAAGTGCTCAATTTTATGTAAATCAATGCGAC
AAGTAAGACAAGCAAGCGTTTACGAGTTGTTAGATGGATATGATTTGCAACAAGCGATTAAAGAAGCGAACGGAGAATGT
GTTACGTACAAACATGAAACATTAGAATCTTCGATACGAAAAGGGTATGCTTTAGGATTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.637

100

0.576

Multiple sequence alignment