Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW25_RS20145 Genome accession   NZ_CP036111
Coordinates   3983000..3983434 (-) Length   144 a.a.
NCBI ID   WP_000811503.1    Uniprot ID   -
Organism   Bacillus toyonensis strain JAS411     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3978000..3988434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW25_RS20130 (EXW25_20505) - 3979168..3979725 (+) 558 WP_000062081.1 PadR family transcriptional regulator -
  EXW25_RS20135 (EXW25_20510) metE 3980249..3982537 (+) 2289 WP_097954290.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW25_RS20140 (EXW25_20515) - 3982575..3982964 (-) 390 WP_000424021.1 competence protein ComJ -
  EXW25_RS20145 (EXW25_20520) nucA/comI 3983000..3983434 (-) 435 WP_000811503.1 DNA-entry nuclease Machinery gene
  EXW25_RS20150 (EXW25_20525) - 3983673..3984422 (+) 750 WP_000387496.1 DUF3967 domain-containing protein -
  EXW25_RS20155 (EXW25_20530) - 3984461..3985735 (-) 1275 WP_000420365.1 HAMP domain-containing sensor histidine kinase -
  EXW25_RS20160 (EXW25_20535) - 3986047..3986247 (+) 201 WP_000929282.1 hypothetical protein -
  EXW25_RS20165 (EXW25_20540) - 3986366..3987529 (+) 1164 WP_097954291.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=346886 EXW25_RS20145 WP_000811503.1 3983000..3983434(-) (nucA/comI) [Bacillus toyonensis strain JAS411]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAGDRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=346886 EXW25_RS20145 WP_000811503.1 3983000..3983434(-) (nucA/comI) [Bacillus toyonensis strain JAS411]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATTGATCGCGGTGGTGCTGGAGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGCTATGATCGTGATGAATGGCCCATGGCGATGTGTAAAGAAGGCGGAAA
AGGGGCGCATATTGAATATATAAGTCCAGCGGATAATCGCGGAGCAGGTTCTTGGGTAGGAAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(50-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment