Detailed information NTG0346794

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: GII85_RS08475
• Genome accession: NZ_CP045817
• Coordinates: 1634283..1635272 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis strain P5_B1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1629283..1640272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GII85_RS08460 (GII85_08460)	acpP	1629480..1629713 (+)	234	WP_003154310.1	acyl carrier protein	-
GII85_RS08465 (GII85_08465)	rncS	1629853..1630602 (+)	750	WP_003232030.1	ribonuclease III	-
GII85_RS08470 (GII85_08470)	smc	1630703..1634263 (+)	3561	WP_041850837.1	chromosome segregation protein SMC	-
GII85_RS08475 (GII85_08475)	pilA	1634283..1635272 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
GII85_RS08480 (GII85_08480)	sivC	1635308..1635793 (-)	486	WP_041850836.1	Ig-like domain-containing protein	-
GII85_RS08485 (GII85_08485)	srpM	1635971..1636303 (+)	333	WP_003238590.1	putative DNA-binding protein	-
GII85_RS08490 (GII85_08490)	ffh	1636317..1637657 (+)	1341	WP_014113771.1	signal recognition particle protein	-
GII85_RS08495 (GII85_08495)	rpsP	1637763..1638035 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
GII85_RS08500 (GII85_08500)	khpA	1638035..1638280 (+)	246	WP_041850835.1	KH domain-containing protein	-
GII85_RS08505 (GII85_08505)	ylqD	1638402..1638788 (+)	387	WP_014476793.1	YlqD family protein	-
GII85_RS08510 (GII85_08510)	rimM	1638793..1639317 (+)	525	WP_041850834.1	ribosome maturation factor RimM	-
GII85_RS08515 (GII85_08515)	trmD	1639314..1640045 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=346794 GII85_RS08475 WP_003232026.1 1634283..1635272(+) (pilA) [Bacillus subtilis strain P5_B1]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=346794 GII85_RS08475 WP_003232026.1 1634283..1635272(+) (pilA) [Bacillus subtilis strain P5_B1]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAACTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCCCATAAAATGAAACAAGAAGGAAAATCTGTCGTACTTGCCGCCGGAGACACTTTCAGA
GCGGGAGCCATTGAACAACTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCCGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGTCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAGTTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAGGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGATCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401