Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW48_RS20750 Genome accession   NZ_CP036101
Coordinates   4114866..4115300 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus wiedmannii strain JAS28/2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4109866..4120300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW48_RS20730 (EXW48_21020) - 4110263..4110820 (+) 558 WP_000062072.1 PadR family transcriptional regulator -
  EXW48_RS20735 (EXW48_21025) - 4111012..4111443 (-) 432 WP_046957439.1 NUDIX hydrolase -
  EXW48_RS20740 (EXW48_21030) metE 4112114..4114402 (+) 2289 WP_001007590.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW48_RS20745 (EXW48_21035) comJ 4114440..4114829 (-) 390 WP_215562060.1 competence protein ComJ -
  EXW48_RS20750 (EXW48_21040) nucA/comI 4114866..4115300 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  EXW48_RS20755 (EXW48_21045) - 4115537..4116286 (+) 750 WP_000387495.1 DUF3967 domain-containing protein -
  EXW48_RS20760 (EXW48_21050) kinB 4116339..4117613 (-) 1275 WP_000420378.1 sporulation sensor histidine kinase KinB -
  EXW48_RS20765 (EXW48_21055) - 4117923..4118123 (+) 201 WP_215562061.1 hypothetical protein -
  EXW48_RS20770 (EXW48_21060) - 4118247..4119410 (+) 1164 WP_046957441.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=346787 EXW48_RS20750 WP_000811502.1 4114866..4115300(-) (nucA/comI) [Bacillus wiedmannii strain JAS28/2]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=346787 EXW48_RS20750 WP_000811502.1 4114866..4115300(-) (nucA/comI) [Bacillus wiedmannii strain JAS28/2]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCATATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCTATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTAAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment