Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   EXW48_RS08030 Genome accession   NZ_CP036101
Coordinates   1588970..1589572 (-) Length   200 a.a.
NCBI ID   WP_000155596.1    Uniprot ID   A0A2A8DKZ6
Organism   Bacillus wiedmannii strain JAS28/2     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1583970..1594572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW48_RS08010 (EXW48_08035) dnaD 1584297..1585004 (+) 708 WP_000728534.1 DNA replication protein DnaD -
  EXW48_RS08015 (EXW48_08040) nth 1585020..1585667 (+) 648 WP_046958931.1 endonuclease III -
  EXW48_RS08020 (EXW48_08045) - 1585671..1586189 (+) 519 WP_046958932.1 YpoC family protein -
  EXW48_RS08025 (EXW48_08050) - 1586213..1588903 (-) 2691 WP_215562927.1 PBP1A family penicillin-binding protein -
  EXW48_RS08030 (EXW48_08055) recU 1588970..1589572 (-) 603 WP_000155596.1 Holliday junction resolvase RecU Machinery gene
  EXW48_RS08035 (EXW48_08060) - 1589676..1590701 (+) 1026 WP_002108635.1 DUF2515 domain-containing protein -
  EXW48_RS08040 - 1591011..1591187 (-) 177 WP_080345072.1 phage portal protein -
  EXW48_RS08045 (EXW48_08065) - 1591273..1591515 (+) 243 WP_046955206.1 hypothetical protein -
  EXW48_RS08050 (EXW48_08070) - 1591531..1591875 (+) 345 WP_000872967.1 YppE family protein -
  EXW48_RS08055 (EXW48_08075) - 1591901..1592092 (-) 192 WP_000242661.1 YppF family protein -
  EXW48_RS08060 (EXW48_08080) - 1592270..1592893 (+) 624 WP_215562928.1 YppG family protein -
  EXW48_RS08065 (EXW48_08085) - 1593049..1593459 (+) 411 WP_046955205.1 CotD family spore coat protein -
  EXW48_RS08070 (EXW48_08090) - 1593516..1594070 (+) 555 WP_000862919.1 DUF1273 domain-containing protein -
  EXW48_RS08075 (EXW48_08095) gpsB 1594168..1594506 (+) 339 WP_000622425.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23371.65 Da        Isoelectric Point: 9.4276

>NTDB_id=346777 EXW48_RS08030 WP_000155596.1 1588970..1589572(-) (recU) [Bacillus wiedmannii strain JAS28/2]
MTIRYPNGKRYNQASQPHKTPIKKHTYSNRGMSLEEELNETNEYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKRVIAHDGIAFVIIKFTLFDELYLLDAKHIIAFWNRQ
NTGGRKSITKEEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=346777 EXW48_RS08030 WP_000155596.1 1588970..1589572(-) (recU) [Bacillus wiedmannii strain JAS28/2]
ATGACCATTCGTTACCCAAACGGAAAACGGTACAATCAAGCTTCACAACCTCATAAAACACCAATTAAAAAACATACTTA
TAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATGAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCGAAAGAAACAAAAAATAA
AACCAGCTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCGAGTAATCGCTCATGATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAACTTTATTTACTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGGAAATCGATTACGAAAGAAGAAATAGAAGAGCATGGATCTTTATTATCATGCGGTTATCACCCTCG
GATTGATTATATCCGTGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8DKZ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63

100

0.63

Multiple sequence alignment