Detailed information NTG0346701

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: EXW54_RS25475
• Genome accession: NZ_CP036094
• Coordinates: 4997425..4998774 (-)
• Length: 449 a.a.
• NCBI ID: WP_098812171.1
• Uniprot ID: -
• Organism: Bacillus toyonensis strain JAS22/1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4992425..5003774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EXW54_RS25455 (EXW54_26055)	secA	4992754..4995261 (-)	2508	WP_000579398.1	preprotein translocase subunit SecA	-
EXW54_RS25460 (EXW54_26060)	raiA	4995536..4996078 (-)	543	WP_000671188.1	ribosome-associated translation inhibitor RaiA	-
EXW54_RS25465 (EXW54_26065)	cspC	4996397..4996594 (-)	198	WP_002045823.1	cold shock protein CspC	-
EXW54_RS25470 (EXW54_26070)	-	4996721..4997425 (-)	705	WP_016107242.1	ComF family protein	-
EXW54_RS25475 (EXW54_26075)	comFA	4997425..4998774 (-)	1350	WP_098812171.1	ATP-dependent helicase ComFA	Machinery gene
EXW54_RS25480 (EXW54_26080)	-	4998901..5000295 (-)	1395	WP_016107244.1	C40 family peptidase	-
EXW54_RS25485 (EXW54_26085)	-	5000456..5000770 (-)	315	WP_000400852.1	helix-turn-helix domain-containing protein	-
EXW54_RS25490 (EXW54_26090)	-	5000942..5001784 (-)	843	WP_000844774.1	DegV family protein	-
EXW54_RS25495 (EXW54_26095)	-	5002023..5002658 (+)	636	WP_098623981.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 50999.76 Da
• Isoelectric Point: 9.4364

>NTDB_id=346701 EXW54_RS25475 WP_098812171.1 4997425..4998774(-) (comFA) [Bacillus toyonensis strain JAS22/1]
MLSGKQLLLEELSTDVRDNLDDLKKKGEVVCVQGVKNKASTYMCQRCGNIAQRLFSSFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAEKEGEMDVNPLQWKGNLSTGQELAAQGVMEAVKQKESFFIWAVCGAGKTEMLFYGIAEALQKGERVCIAT
PRTDVVLELAPRLKEVFPYINVAALYGRSLDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVKQAM
KEKAACIYLTATPDEEWKRNLRKGKQKGIVVSGRYHRHPLPVPKFCCCGNWKKSLINKRIPQVLLQWLKVYLNKKHPIFL
FVPHVRYIEEMSRLLKLLDDRVAGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGTEEEIFSESALV
QIAGRVGRSFEEPYGEVIYFHYGKTEAMVRAKKHIRSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=346701 EXW54_RS25475 WP_098812171.1 4997425..4998774(-) (comFA) [Bacillus toyonensis strain JAS22/1]
ATGTTATCTGGGAAACAGTTATTATTAGAAGAACTCTCTACAGACGTACGGGATAATTTAGATGATTTGAAGAAGAAGGG
AGAGGTCGTATGTGTACAAGGTGTAAAAAATAAGGCTTCTACATATATGTGTCAGCGTTGCGGAAATATAGCACAAAGAC
TCTTTTCATCATTTTTGTGTAAAAGGTGTAGTAAAGTCTGCACATATTGCCGGAAGTGCATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGAATTGCCGAAAAAGAGGGAGAAATGGATGTAAACCCGTTACAGTGGAAAGGAAATTT
ATCTACTGGCCAGGAGTTGGCTGCGCAAGGTGTTATGGAGGCTGTTAAACAAAAAGAATCGTTTTTTATTTGGGCTGTGT
GCGGCGCTGGAAAAACAGAAATGTTGTTTTATGGTATTGCAGAGGCACTTCAAAAAGGAGAAAGGGTTTGTATTGCAACG
CCGAGAACGGATGTTGTACTTGAATTAGCACCGAGATTGAAAGAAGTATTTCCTTATATAAATGTAGCTGCTTTATACGG
TAGGAGTTTAGATCGGGAAAAAGATGCAGCGTTAGTCGTTGCGACCACCCATCAGCTATTGCGTTATTATAGAGCGTTTC
ATGTCATGATTGTAGATGAAATAGATGCCTTTCCATACCATGCAGATCAAATGTTGCAGTATGCGGTAAAACAAGCGATG
AAAGAGAAAGCGGCATGTATTTATTTAACTGCAACTCCAGATGAAGAGTGGAAGCGTAACCTTAGAAAGGGGAAACAAAA
AGGTATCGTTGTTTCTGGACGGTATCACCGTCATCCGTTGCCAGTCCCCAAATTTTGCTGTTGCGGAAACTGGAAGAAAA
GCCTCATCAATAAAAGAATTCCTCAAGTTTTACTACAATGGTTAAAAGTATACTTAAATAAAAAGCACCCCATTTTTTTA
TTTGTTCCCCATGTGCGATATATAGAAGAAATGAGTCGGTTATTGAAGTTGTTAGACGATAGAGTTGCCGGTGTGCATGC
AGAAGATCCAATGAGAAAAGAGAAAGTTGCAGCTTTCAGAAAGGGAGAAATTCCTTTATTAGTTACAACGACGATTTTGG
AGAGAGGAGTAACGGTGAAAAATTTGCAAGTTGCGGTTTTAGGGACGGAAGAAGAAATATTTTCAGAAAGTGCACTCGTA
CAAATTGCAGGCCGGGTAGGTCGTAGTTTTGAAGAGCCGTATGGAGAGGTCATTTATTTTCATTATGGTAAGACAGAGGC
GATGGTGCGCGCGAAAAAACACATTCGAAGTATGAACAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

• Type III restriction enzyme, res subunit (ResIII) (106-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50	98.441	0.492
comFA	Latilactobacillus sakei subsp. sakei 23K	40.695	89.755	0.365