Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII86_RS13190 Genome accession   NZ_CP045816
Coordinates   2468039..2468449 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain P5_B2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2463039..2473449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII86_RS13160 (GII86_13160) - 2463125..2463265 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII86_RS13165 (GII86_13165) yqeF 2463571..2464302 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  GII86_RS13170 (GII86_13170) cwlH 2464554..2465306 (-) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII86_RS13175 (GII86_13175) yqeD 2465493..2466119 (+) 627 WP_046160610.1 TVP38/TMEM64 family protein -
  GII86_RS13180 (GII86_13180) gnd 2466139..2467032 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII86_RS13185 (GII86_13185) yqeB 2467284..2468006 (+) 723 WP_014480321.1 hypothetical protein -
  GII86_RS13190 (GII86_13190) nucA/comI 2468039..2468449 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII86_RS13195 (GII86_13195) - 2468645..2468992 (+) 348 Protein_2580 sigma-70 family RNA polymerase sigma factor -
  GII86_RS13200 (GII86_13200) spoIVCA 2469023..2470483 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  GII86_RS22205 - 2470441..2470619 (-) 179 Protein_2582 hypothetical protein -
  GII86_RS13205 (GII86_13205) arsC 2470974..2471393 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GII86_RS13210 (GII86_13210) acr3 2471405..2472445 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  GII86_RS13215 (GII86_13215) arsK 2472468..2472908 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GII86_RS13220 (GII86_13220) arsR 2472967..2473284 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=346678 GII86_RS13190 WP_009967785.1 2468039..2468449(-) (nucA/comI) [Bacillus subtilis strain P5_B2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=346678 GII86_RS13190 WP_009967785.1 2468039..2468449(-) (nucA/comI) [Bacillus subtilis strain P5_B2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment