Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   EXW46_RS14490 Genome accession   NZ_CP036085
Coordinates   2780341..2780775 (+) Length   144 a.a.
NCBI ID   WP_002014817.1    Uniprot ID   -
Organism   Bacillus mycoides strain JAS12/5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2775341..2785775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW46_RS14470 (EXW46_14740) - 2776243..2777400 (-) 1158 WP_215576535.1 aminotransferase A -
  EXW46_RS14475 (EXW46_14745) - 2777518..2777718 (-) 201 WP_000929276.1 hypothetical protein -
  EXW46_RS14480 (EXW46_14750) - 2778028..2779302 (+) 1275 WP_002014814.1 HAMP domain-containing sensor histidine kinase -
  EXW46_RS14485 (EXW46_14755) - 2779361..2780104 (-) 744 WP_002033817.1 DUF3967 domain-containing protein -
  EXW46_RS14490 (EXW46_14760) nucA/comI 2780341..2780775 (+) 435 WP_002014817.1 DNA-entry nuclease Machinery gene
  EXW46_RS14495 (EXW46_14765) comJ 2780815..2781204 (+) 390 WP_002088359.1 competence protein ComJ -
  EXW46_RS14500 (EXW46_14770) metE 2781245..2783533 (-) 2289 WP_002014819.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  EXW46_RS14505 (EXW46_14775) - 2784075..2784632 (-) 558 WP_060749077.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16194.59 Da        Isoelectric Point: 8.4331

>NTDB_id=346598 EXW46_RS14490 WP_002014817.1 2780341..2780775(+) (nucA/comI) [Bacillus mycoides strain JAS12/5]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKEGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=346598 EXW46_RS14490 WP_002014817.1 2780341..2780775(+) (nucA/comI) [Bacillus mycoides strain JAS12/5]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACGGATGCTATAAAAGAAGGACATTCGGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.027

78.472

0.542

Multiple sequence alignment